Genomes

Genomes is taught at the university of Leicester as a second year undergraduates course.
Level: Undergraduate
UK CATS System: 20 Credits

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Course Components

Course Description

In this module, students learn the basic techniques of modern molecular biology and see how they are being used to investigate the organisation, evolution and maintenance of integrity of their genomes. This course provides the students with "hands on" experience of many of the relevant techniques. In order to be able to get a full view of genomes in general, this course considers a wide range of genomes, including those of bacteria, yeast, drosophila and humans.

Technical Requirements

This module requires a reasonable amount of preparation and equipment. A full list of all the technical information needed to run the practical elements of this course will soon be available as a downloadable file.

Learning Outcomes

At the completion of the module, students should be able to:

  • describe how prokaryotic and eukaryotic genomes are organised
  • discuss the mechanisms operating to influence genome organisation
  • describe the basic processes of DNA replication, recombination and repair
  • carry out a variety of molecular techniques and use them in the design of experiments to address problems relating to genome research
  • work as a group to research problems
  • organise written and oral presentation of research findings

 

Course Map

Term Week Lecture # Lecture Title Tutorials Practical 1: Cloning Random Segments of Human DNA into a Plasmid Practical 2: Restriction Mapping of the λ Phage Genome
1   Module Introduction      
2 1 Prokaryotic Genomes- An Overview Isolation and analysis of yeast genes   Session 1: Preparation of competent cells and transformation with plasmid DNA  
   2 Cloning       
   3 Cloning       
 4 Organisation of Eukaryotic Genomes   Session 2: Examination of transformants  
   5 Organisation of Eukaryotic Genomes   Session 3: Preparation of plasmid DNAs; PCR amplification of plasmid inserts; Digestion of plasmid DNAs  
   6 Organisation of Eukaryotic Genomes  
 
 4  7 Analysis of Protein Coding Genes Cloning and expression of a medically important protein Session 4: Electrophoresis gels of digested plasmid DNA Session 1: Begin construction of a λ phage restriction map
   8 Analysis of Protein Coding Genes   Session 5: Analysis of gels: Southern blotting of plasmid DNA gel  
   9 Analysis of Protein Coding Genes      
 5  10 Analysis of Protein Coding Genes   Session 6: Results of Southern blot: Gels of PCR products  
   11 Analysis of Protein Coding Genes   Session 7: Mapping of plasmid clone DNA sequences in the human genome Session 2: Complete construction of the λ phage restriction map
   12 Analysis of Protein Coding Genes      
 6  13 Replication, Recombination and Repair Computer analysis of a DNA sequence     
   14 Replication, Recombination and Repair Building DNA models    
   15 Replication, Recombination and Repair      
 16 Molecular and Genome Evolution      
   17 Molecular and Genome Evolution      
   18 Molecular and Genome Evolution      
 8  19 Microbial Genomics      
   20

Microbial Genomics 

     
   21 Microbial Genomics       
   22 Microbial Genomics      
   23 Introduction to the the Analysis of the Human Genome        
   24 Introduction to the the Analysis of the Human Genome       
 10  25 Introduction to the the Analysis of the Human Genome        
   26 Introduction to the the Analysis of the Human Genome        
   27      Practicals Feedback  Practicals Feedback
           

Sample Exam Papers

These are available to download as .docx, rtf or pdf; however, answers are not available.


.docx .rtf .pdf .zip NOTES
This is a zip folder with all the exam papers.       Download arrow This zip folder contains all files.
1 Exam paper for 2005 Download arrow Download arrow Download arrow   Past examination questions for 2005
2 Exam paper for 2006 Download arrow Download arrow Download arrow   Past examination questions for 2006
3 Exam paper for 2006 Download arrow Download arrow Download arrow   Past examination questions for 2006

 

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