Publications

Publications

2015

Flayhan A, Bergé C, Baïlo N, Doublet P, Bayliss R, and Terradot L. The structure of Legionella pneumophila LegK4 type four secretion system (T4SS) effector reveals a novel dimeric eukaryotic-like kinase. Scientific Reports 14602 doi:10.1038/srep14602

Burgess SG, Peset I, Joseph N, Cavazza T, Vernos I, Pfuhl M, Gergely F, Bayliss R Aurora-A-dependent control of TACC3 influences the rate of mitotic spindle assembly. PLOS Genetics. DOI: 10.1371/journal.pgen.1005345

Rogerson D, Sachdeva A, Wang K, Haq T, Kazlauskaite A, Muqit MMK, Fry AM, Bayliss R, Chin J. Efficient and site-specific incorporation of phosphoserine and its non-hydrolyzable analog into recombinant proteins. Nat. Chem. Biol. (in press). doi:10.1038/nchembio.1823

Burgess SG, Bayliss R, Pfuhl MP. Solution NMR assignment of the cryptic sixth TOG domain of minispindles. Biomolecular NMR Assignments (in press).

Bayliss R, Haq T, Yeoh S. The Ys and wherefores of protein kinase autoinhibition. BBA Proteins and Proteomics doi:10.1016/j.bbapap.2015.04.025

O’Regan L, Sampson J, Richards MW, Knebel A, Roth D, Hood FE, Straube A, Royle SJ, Bayliss R, Fry AM. Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression. J. Cell. Biol. doi:10.1083/jcb.201409151.

Joshi A, Newbatt Y, McAndrew PC, Stubbs M, Burke R, Richards MW, Bhatia C, Caldwell JJ, McHardy T, Collins I, Bayliss R. Molecular mechanisms of human IRE1 activation through dimerization and ligand binding. Oncotarget 5. full text OA

Richards MW, O’Regan L, Roth D, Montgomery JM, Straube A, Fry AM, Bayliss R. Microtubule association of EML proteins and the EML4-ALK variant 3 oncoprotein require an N-terminal trimerization domain. Biochem. J. doi:10.1042/BJ20150039

Burgess SG, Bayliss R. The structure of C290A:C393A Aurora A provides structural insights into kinase regulation. Acta Cryst. F 71 doi:10.1107/S2053230X15002290

Gutiérrez-Caballero C, Burgess SG, Bayliss R, and Royle SJ. TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biology Open 4:170-179. doi:10.1242/bio.201410843

2014

Rowan F, Richards M, Widya M, Bayliss R, Blagg J. Diverse functionalization of Aurora-A kinase at specified surface and buried sites by native chemical modification. PLoS One. 2014 Aug 5;9(8):e103935. doi: 10.1371/journal.pone.0103935

Fry AM, Leaper MJ, Bayliss R. The primary cilium: Guardian of organ development and homeostasis. Organogenesis 10:62-68. doi: 10.4161/org.28910.

Richards MW, Law EP, Rennalls LP, Busacca S, O'Regan L, Fry AM, Fennell DA, Bayliss R. Crystal structure of EML1 reveals the basis for Hsp90 dependence of oncogenic EML4-ALK by disruption of an atypical b-propeller domain. Proc. Natl. Acad. Sci. USA 111:5195-200. doi: 10.1073/pnas.1322892111.

Bojar D, Martinez J, Santiago J, Rybin V, Bayliss R, Hothorn M. Crystal structures of the phosphorylated BRI1 kinase domain and implications for brassinosteroid signal initiation. Plant J. doi: 10.1111/tpj.12445.

 

2013

Bavetsias V, Faisal A, Crumpler S, Brown N, Kosmopoulou M, Joshi A, Atrash B, Pérez-Fuertes Y, Schmitt JA, Boxall KJ, Burke R, Sun C, Avery S, Bush K, Henley A, Raynaud FI, Workman P, Bayliss R, Linardopoulos S, Blagg J (2013). Aurora Isoform Selectivity: Design and Synthesis of Imidazo[4,5-b]pyridine Derivatives as Highly Selective Inhibitors of Aurora-A Kinase in Cells. J Med Chem. 56:9122-35

Rowan FC, Richards M, Bibby RA, Thompson A, Bayliss R, Blagg J (2013) Insights into Aurora-A kinase activation using unnatural amino acids incorporated by chemical modification. ACS Chem. Biol. (DOI 10.1021/cb400425t).

Dodson CA, Haq T, Yeoh S, Fry AM, Bayliss R** (2013) The structural mechanisms that underpin mitotic kinase activation Biochem.Soc.Trans. 41:1037-1041. Review article

Hood FE, Williams SJ, Burgess SG, Richards MW, Roth D, Straube A, Pfuhl M, Bayliss R, Royle SJ (2013) Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding J. Cell. Biol. 202:463-478.

Dodson CA, Yeoh S, Haq T, Bayliss R (2013) A Kinetic Test Characterizes Kinase Intramolecular and Intermolecular Autophosphorylation Mechanisms. Sci Signal. 6:ra54.

Summary Abstract Reprint Full Text

 

2012

Bayliss R,  Fry AM, Haq T, Yeoh S (2012) On the molecular mechanisms of mitotic kinase activation. Open Biology doi:10.1098/rsob.120136. Review article.

Fry AM, O'Regan L, Sabir SR, Bayliss R (2012) Cell cycle regulation by the NEK family of protein kinases. J. Cell. Sci. 125:4423-33. Review article.

Bavetsias V, Crumpler S, Sun C, Avery S, Atrash B, Faisal A, Moore AS, Kosmopoulou M, Brown N, Sheldrake PW, Bush K, Henley A, Box G, Valenti M, de Haven Brandon A, Raynaud FI, Workman P, Eccles SA, Bayliss R, Linardopoulos S, Blagg J (2012) Optimization of Imidazo[4,5-b]pyridine-Based Kinase Inhibitors: Identification of a Dual FLT3/Aurora Kinase Inhibitor as an Orally Bioavailable Preclinical Development Candidate for the Treatment of Acute Myeloid Leukemia. J. Med. Chem. 55:8721-34.

Innocenti P, Cheung K-MJ, .Solanki S, Mas-Droux C, Rowan F, Yeoh S, Boxall K, Westlake M, Pickard L, Hardy T, Baxter JE, Aherne GW, Bayliss R, Fry AM, Hoelder S (2012) Design of potent and selective hybrid inhibitors of the mitotic kinase Nek2: SAR, structural biology and cellular activity. J.Med.Chem. 55:3228-41.

Dodson CA, Bayliss R (2012) Activation of Aurora-A kinase by protein partner binding (TPX2) and phosphorylation are independent and synergistic. J.Biol.Chem. 287:1150-7.

Zalli D, Bayliss R, Fry AM (2012) The Nek8 protein kinase, mutated in the human cystic kidney disease nephronophthisis, is both activated and degraded during ciliogenesis. Hum. Mol. Gen. 21:1155-71.

 

2009-2011

Solanki S, Innocenti P, Mas-Droux C, Boxall K, Barillari C, van Montfort RLM, Aherne GW, Bayliss R, and Hoelder S (2011) Benzimidazole Inhibitors Induce a DFG-Out Conformation of Never in Mitosis Gene A-Related Kinase 2 (Nek2) without Binding to the Back Pocket and Reveal a Nonlinear Structure−Activity Relationship. J. Med. Chem. 54: 1626-1639.

Oberoi J, Richards MW, Crumpler S, Brown N, Blagg J, Bayliss R (2010) Structural basis of poly(ADP-ribose) recognition by the multi-zinc binding domain of checkpoint with forkhead-associated and RING domains (CHFR). J. Biol. Chem. 285: 39348-3935.

Whelligan DK, Solanki S, Taylor D, Thomson DW, Cheung K-MJ, Boxall K, Mas-Droux C, Barillari C, Burns S, Grummitt CG, Collins I, van Monfort RLM, Aherne W, Bayliss R, Hoelder S (2010) Aminopyrazine inhibitors binding to an unusual inactive conformation of the mitotic kinase Nek2: SAR and structural characterization. J. Med. Chem. 53(21):7682-98.

Bouloc N, Large JM, Kosmopoulou M, Sun C, Faisal A, Matteucci M, Reynisson J, Brown N, Atrash B, Blagg J, McDonald E, Linardopoulos S, Bayliss R, Bavetsias V (2010) Structure-based design of imidazo[1,2-a]pyrazine derivatives as selective inhibitors of Aurora-A kinase in cells. Bioorg. Med. Chem. Lett. 20(20):5988-93

Bavetsias V, Large JM, Sun C, Bouloc N, Kosmopoulou M, Matteucci M, Wilsher NE, Martins V, Reynisson J, Atrash B, Faisal A, Urban F, Valenti M, Brandon AH, Box G, Raynaud FI, Workman P, Eccles SA, Bayliss R, Blagg J, Linardopoulos S, McDonald E (2010) Imidazo[4,5-b]pyridine derivatives as inhibitors of Aurora kinases: lead optimisation studies towards the identification of an orally bioavailable preclinical development candidate. J. Med. Chem. 53(14):5213-28

Dodson CA, Kosmopoulou M, Richards MW, Atrash B, Bavetsias V, Blagg J, Bayliss R** (2010) Crystal structure of an Aurora-A mutant that mimics Aurora-B bound to MLN8054: insights into selectivity and drug design. Biochem. J. 427(1):19-28

Richards MW, Leung J, Roe SM, Chen J, Bayliss R** (2010) A pocket on the surface of the N-terminal BRCT domain of Mcph1 is required to prevent abnormal chromosome condensation. J. Mol. Biol. 395(5):908-15

Richards MW, O’Regan L, Mas-Droux C, Blot JMY, Cheung J, Hoelder S, Fry AM, Bayliss R** (2009) An auto-inhibitory tyrosine motif in the cell cycle-regulated Nek7 kinase is released through binding of Nek9. Mol. Cell. 36(4):560-70

Bibby R, Tang C, Faisal A, Drosopoulos K, Lubbe S, Houlston R, Bayliss R, Linardopoulos S (2009) A cancer associated Aurora-A mutant is mislocalised and misregulated due to loss of interaction with TPX2. J. Biol. Chem. 284(48):33177-84

Westwood I, Cheary D-M, Baxter JE, Richards MW, van Montfort R, Fry AM, Bayliss R** (2009) Insights into the conformational variability and regulation of human Nek2 kinase. J. Mol. Biol. 386(2):476-85

Jakubowski SJ, Kerr J, Garza I, Krishnamoorthy V, Bayliss R, Waksman G, Christie PJ (2009) Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesis. Mol. Microbiol. 71(3):779-94

 

pre 2009

Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Hass R, Waksman G (2007) Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase. EMBO J. 26(23):4926-34

Richter S, Lindenstrauß U, Lücke C, Bayliss R, Brüser T (2007) Functional Tat transport of unstructured, small, hydrophilic proteins. J. Biol. Chem. 282(46):33257-64

Bayliss R*, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll P, Waksman G (2007) NMR Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system. Proc. Natl. Acad. Sci. USA 104(5): 1673-8

Paschos A, Patey G, Sivanesan D, Gao C, Bayliss R, Waksman G, O'Callaghan D, Baron C (2006) Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proc. Natl. Acad. Sci. USA 103(19): 7252-7

Hare S, Bayliss R, Baron C and Waksman G (2006) A large domain swap in the VirB11 ATPase of Brucella suis leaves the hexameric assembly intact. J. Mol. Biol. 360(1):56-66

Harris R, Bayliss R, Waksman G, Driscoll, P (2006) Backbone 1H, 13C, and 15N resonance assignments of the complex formed between TraN (a VirB7 homologue) and the C-terminal domain of TraO (a VirB9 homologue) from the conjugative plasmid pKM101. Journal of Biomolecular NMR 36:suppl. 1:31s

Terradot L, Bayliss R*, Oomen C, Leonard G, Baron C, Waksman G (2005) Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori. Proc. Natl. Acad. Sci. USA 102(12):4596-601

Bayliss R*, Sardon T, Ebert J, Lindner D, Vernos I, Conti E (2004) Determinants for Aurora-A activation and Aurora-B discrimination by TPX2. Cell Cycle. 3(4): 404-407

Bayliss R*, Sardon T, Vernos I, Conti E (2003) Structural basis of Aurora-A activation by TPX2 at the mitotic spindle. Mol.Cell. 12(4): 851-62

Bayliss R*, Littlewood T, Strawn LA, Wente SR, Stewart M (2002) GLFG and FxFG nucleoporins bind to overlapping sites on Importin-b. J. Biol. Chem. 277(52): 50597-606

Bayliss R*, Leung SW, Baker RP, Quimby BB, Corbett AH, Stewart M (2002) Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats. EMBO J. 21(12): 2843-53

Quimby BB, Leung SW, Bayliss R, Harreman MT, Thirumala G, Stewart M, Corbett AH (2001) Functional analysis of the hydrophobic patch on NTF2 involved in interactions with the nuclear pore in vivo. J. Biol. Chem. 276(42): 38820-9

Stewart M, Baker RP, Bayliss R, Clayton L, Grant RP, Littlewood T, Matsuura Y (2001) Molecular mechanism of translocation through nuclear pore complexes during nuclear protein import. FEBS Lett. 498(2-3): 145-9. Review Article

Bayliss R*, Kent HM, Corbett AH, Stewart M (2000) Crystallization and initial diffraction characterization of complexes of FxFG nucleoporin repeats with nuclear transport factors. J. Struct. Biol. 131(3): 240-7

Bayliss R*, Littlewood T, Stewart M (2000) Structural basis for the interaction between FxFG nucleoporin repeats and Importin-b in nuclear trafficking. Cell 102(1): 99-108

Bayliss R*, Corbett AH, Stewart M (2000) The molecular mechanism of transport of macromolecules through Nuclear Pore Complexes. Traffic 1(6): 448-56. Review Article

Bayliss R*, Ribbeck K, Akin D, Kent HM, Feldherr CM, Görlich D, Stewart M (1999) Interaction between NTF2 and xFxFG-containing nucleoporins is required to mediate nuclear import of RanGDP. J. Mol. Biol. 293(3): 579-93

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