Nucleic Acids Research Breakthrough article describes novel mechanisms associated with mammalian splicing enhancers

A recent article published in Nucleic Acids Research, co-authored by Prof Ian Eperon and Dr Andrew Hudson (University of Leicester) and Dr Glenn Burley (University of Strathclyde) has been designated a Breakthrough article by the journal editors. The paper describes studies that provide important new insight into the mechanisms that regulate pre-mRNA splicing in mammalian cells. The study exploits single molecule techniques (multi-colour, co-localization, fluorescence imaging) to quantify stable binding of proteins to pre-mRNA in a nuclear extract that supports splicing, and chemical biology to dissect the subsequent activation process. The research project was supported by the Leverhulme Trust.

Reviewers and editors familiar with the work have stated that the study "re-addresses long-standing open questions”; that the results are "unexpected and contrast with the interpretation of (historical) data"; utilise “approaches that would have been impossible twenty years ago "; and that the work "provides interesting new insights that could not have been obtained by conventional ensemble methods and that will be of wide interest in the field of splicing regulation and its mechanisms."

"The mechanisms of a mammalian splicing enhancer"

(Andrew M. Jobbins, Linus Reichenbach, Christian M. Lucas, Andrew J. Hudson, Glenn Burley and Ian C. Eperon; Nucleic Acids Research, Volume 46, Issue 5, 16 March 2018, Pages 2145–2158, https://doi.org/10.1093/nar/gky056)

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