Richard J Mbasu



Richard J Mbasu



MSc Cancer Cell and Molecular Biology, 2011, University of Leicester
BSc Medical Sciences, 2010, University of Leeds
Research Area Proteomics
Mass Spectrometry
Research Aim Investigate biomarkers of diastolic heart failure (DHF) in human plasma.
Overview I am pursuing identification of biomarkers for diastolic heart failure (DHF), which is as common a cause of dyspnoea and effort intolerance as systolic heart failure (SHF), but which currently is under-diagnosed because it requires sophisticated imaging techniques to do so. The need for readily available blood tests to diagnose or rule out DHF will become even more urgent as therapies are developed which target this form of heart failure; hence this work will be of particular interest to Industry. We will identify patients with DHF (and controls) using state-of-the-art imaging techniques (tissue Doppler echo and MRI) available in the LCBRU and will use our expertise in spectrometry and protein analysis to identify plasma peptides that differentiate patients with DHF, focusing especially on extracellular matrix (ECM) proteins and degradation products which are likely to be disrupted in DHF.



Mbasu RJ*, Heaney LM*, Molloy BJ*, Hughes CJ*, Ng LL, Vissers JPC, Langridge JI, Jones DJL. Advances in quadrupole and time-of-flight mass spectrometry for peptide MRM based translational research analysis. Proteomics 2016;16:2206-2220.

Heaney LM*, Jones DJL*, Mbasu RJ, Ng LL, Suzuki T. High mass accuracy assay for trimethylamine N-oxide using stable-isotope dilution with liquid chromatography coupled to orthogonal acceleration time of flight mass spectrometry with multiple reaction monitoring. Analytical and Bioanalytical Chemistry 2016;408:797-804

Mbasu RJ, Hakimi A, Sandhu JK, Heaney LM, Quinn PA, Bhandari SS, Jones DJL, Ng LL. Proteomics of human plasma in diastolic heart failure (DHF) using novel chemical affinity, mixed mode matrix (M3). Heart 2015;101:A7

*equal contribution

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