Histology Facility

 

spleen
Haematoxylin and Eosin (H+E) on human spleen
Providing a comprehensive range of histological techniques. The facility includes a cut-up area, processor/embedder, cryostat and microtomes. Cutting is generally undertaken on standard blocks but megablocks can also be cut. H&E staining is performed on a linear stainer ensuring consistent results as assessed by the NEQAS quality assurance scheme. Immunohistochemistry can be performed using a variety of techniques to fit the researcher's needs.
Project logistics should first be discussed with the lead technician.
Services provided include:

1. Receipt and Sample Preparation

  • Fresh tissue for freezing and nitrogen storage
  • Tissue fixed in 10% Formalin for processing to paraffin wax
  • Production of cytoblocks from provided cells

 

2. Cutting Facility
tonsil 300
Leucocyte Common Antigen (LCA) on human tonsil using Novolink/DAB detection kit

  • Frozen sections of fresh tissue for RNA/DNA extraction (placed into eppendorfs with lysis buffer)
  • Frozen sections of fresh tissue on treated slides for staining or Immunohistochemistry
  • Wax sections on untreated slides for staining or RNA/DNA extraction
  • Wax sections on treated slides for Immunohistochemistry
  • Megablock sections on large slides for hand staining

3. Staining currently available

  • H&E
  • Immunohistochemistry
  • Special stains (e.g. Perls, Elastic-Van Gieson)

4.  Access Diagnostic Archives on your behalf.

 

Location: Level 3, RKCSB, LRI

Academic Lead: John le Quesne

Contact: Angie Gillies (Histology Manager), E-mail: akg8@le.ac.uk, Tel: 0116 252 3235

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