Dr Andrew R. Bottrill

Dr Andrew R. Bottrill

Senior Experimental Officer

Proteomics Facility

Protein and Nucleic Acid Chemistry Laboratory

University of Leicester

+44 (0)116 2231551


Hodgkin Building, Lancaster Rd., Leicester, UK.

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The identification and characterisation of proteins and protein complexes is central to the work carried out in my laboratory. Heading up the University of Leicester Proteomics Facility my group uses cutting-edge analytical technologies in pursuing these goals. Our laboratory is equipped with a number of mass spectrometers including LTQ-Orbitrap-Velos (with ETD) and 4000 Q-Trap.


Protein phosphorylation site analysis is an area of increasing speciality within the Proteomics group, underpinning much of the research carried out by the many Cell Signalling groups at the University of Leicester. Strategies typically involve chromatographic separation using HILIC, ERLIC or Cation Exchange follwed by phosphopeptide enrichment using IMAC or TiO2. The raw power of the LTQ-Orbitrap is utilised for phospho site discovery and the specfic scan features of the 4000 Q-Trap are harnessed for validation.


Subtle changes in protein expression are increasingly key in many of these studies. Quantitative proteomic approaches, both labelled (SILAC and Reporter-Ion) and label-free (SRM and Spectral Counting), are being utilised to elucidate these changes.



List of Publications by Year:

Citation Indices

Hay, J.M.; Jordan, E.S.; Browne, G.J.; Bottrill, A.R.; Prigent, S.A.; Dickens, M.
Transcriptional and Post-Translational Targeting of Myocyte Stress Protein 1 (MS1) by the JNK Pathway in Cardiac Myocytes.
Journal of Molecular Signalling, 2017, http://doi.org/10.5334/1750-2187-12-3

Saini, E.; Zeeshan, M.; Brady, D.; Pandey, R.; Kaiser, G.; Koreny, L.; Kumar, P.; Thakur, V.; Tatiya, S.; Katris, N.J.; Limenitakis, R.S.; Kaur, I.; Green, J.L.; Bottrill, A.R.; Guttery, D.S.; Waller, R.F.; Heussler, V.; Holder, A.A.; Mohmmed, A.; Malhotra, P.; Tewari, R.
Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development.
Scientific Reports, 2017, doi:10.1038/s41598-017-15781-z

Wan, M.W.R.; Browne, G.; Karountzos, A.; Stevens, C.; Alghamdi, Y.; Bottrill, A.R.; Mistry, S.; Smith, E.; Bushel, M.; Pingle, J.H.; Sayan, A.E.; Tulchinsky, E.
Short stretches of rare codons regulate translation of the transcription factor ZEB2 in cancer cells.
Oncogene, 2017, doi: 10.1038/onc.2017.273

Rieck, B.; Degiacomi, G.; Zimmermann, M.; Cascioferro, A.; Boldrin, F.; Lazar-Adler, N.R.; Bottrill, A.R.; le Chevalier, F.; Friugi, W.; Bellinzoni, M.; Lisa, M.-N.; Alzari, P.M.; Nguyen, L.; Brosch, R.; Sauer, U.; Manganelli, R.M.; O'Hare, H.M.
PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis.
PLOS Pathogens, 2017, https://doi.org/10.1371/journal.ppat.1006399

Pinto, E.; Anselmo, M.; Calha, M.; Bottrill, A.R.; Duarte, I.; Andrew, P.; Faleiro, M.L.
The intestinal proteome of diabetic and control children is enriched with different microbial and host proteins.
Microbiology, 2017, doi: 10.1099/mic.0.000412

Mitcheson, D.F.; Bottrill, A. R.; Carr, K.; Coxon, C.R.; Cano, C.; Golding, B.T.; Griffin, R.J.; Fry, A.M.; Doerig, C.; Bayliss, R.; Tobin A.B.
A new tool for the chemical genetic investigation of the Plasmodium falciparum Pfnek-2 NIMA-related kinase.
Malaria Journal, 2016, DOI: 10.1186/s12936-016-1580-3

Zindel, D.; Engel, S.; Bottrill, A. R.; Pin, J. P.; Prézeau, L.; Tobin, A.B.; Krasel, C.; Butcher, A.J.
Identification of key phosphorylation sites in PTH1R which determine arrestin3 binding and fine tune receptor signaling.
Biochemical Journal, 2016, DOI: 10.1042/BCJ20160740

Tsimokha, A. S.; Zaykova, J. J.; Bottrill, A. R.; Barlev, N. A.
Extracellular Proteasomes are Deficient in 19S Subunits as Revealed by iTRAQ Quantitative Proteomics.
Journal of Cellular Physiology, 2016, DOI: 10.1002/jcp.25492

Millard, C. J.; Varma, N.; Saleh, A.; Morris, K.; Watson, P. J.; Bottrill, A. R.; Fairall, L.; Smith, C. J.; Schwabe, J. W.
The structure of the core NuRD repression complex provides insights into its interaction with chromatin.
elife, 2016, 5, e13941

Scott, D.; Garner, T. P.; Long, J.; Strachan, J.; Mistry, S. C.; Bottrill, A. R.; Tooth, D. J.; Searle, M. S.; Oldham, N. J.; Layfield, R.
Mass spectrometry insights into a tandem ubiquitin-binding domain hybrid engineered for the selective recognition of unanchored polyubiquitin.
Proteomics, 2016, doi: 10.1002/pmic.201600067

Butcher, A.J.; Bradley, S.J.; Prihandoko, R.; Brooke, S.M.; Mogg, A.; Bourgognon, J.-M.; Macedo-Hatch, T.; Edwards, J.M.; Bottrill, A.R.; Challiss, R.A.; Broad, L.M.; Felder, C.C.; Tobin, A.B.
An antibody biosensor establishes the activation of the M1 muscarinic acetylcholine receptor during learning and memory.
Journal of Biological Chemistry, 2016, doi: 10.1074/jbc.M115.681726

Alam, M.M.; Solyakov, L.; Bottrill, A.R.; Flueck, C.; Siddiqui, F.A.; Singh, S.; Mistry, S.; Viskaduraki, M.; Lee, K.; Hopp, C.S.; Chitnis, C.E.; Doerig, C.; Moon, R.W.; Green, J.L.; Holder, A.A.; Baker, D.A.; Tobin, A.B.
Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion.
Nature Communications, 2015, 6: 7285, doi:10.1038/ncomms8285

Turapov, O; Lorraine, J.; Jenkins, C.H.; Barthe, P.; McFeely, D.; Forti, F.; Ghisotti, D.; Hesek, D.; Lee, M.; Bottrill, A.R.; Vollmer, W.; Mobashery, S.; Cohen-Gonsaud, M.; Mukamolova, G.V.
The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth.
Open Biology, 2015, 5: 150025, doi: 10.1098/rsob.150025

Stekhoven, F.M.A.H.S; van der Velde, G.; Lee, T.-H.; Bottrill, A.R.
Proteomic study of the brackish water mussel Mytilopsis leucophaeata.
Zoological Studies, 2015, 54:22, doi:10.1186/s40555-014-0081-8

Wright, M.H.; Clough, B.; Rackham, M.D.; Rangachari, K.; Brannigan, J.A.; Grainger, M.; Moss, D.K.; Bottrill, A.R.; Heal, W.P.; Broncel, M.; Serwa, R.A.; Brady, D.; Mann, D.J.; Leatherbarrow, R.J.; Tewari, R.; Wilkinson, A.J.; Holder, A.A.; Tate, E.W.
Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach.
Nature Chemistry, 2014, 6, 112-121

Smith, L.D.; Dickinson, R.L; Lucas,C.M.; Cousins, A.; Malygin, A.A.; Weldon, C.; Perrett, A.J.; Bottrill, A.R.; Searle, M.S.; Burley, G.A.*, Eperon, I.C.
A targeted oligonucleotide enhancer of SMN2 exon 7 splicing forms competing quadruplex and protein complexes in functional conditions.
Cell Reports, 2014, 9, 1-13 DOI: http://dx.doi.org/10.1016/j.celrep.2014.08.051

Patel, J.T.; Bottrill, A.R.; Prosser, S.L.; Jayaraman, S.; Straatman, K.; Fry, A.M.; Shackleton, S.K.
Mitotic phosphorylation of SUN1 loosens its connection with the nuclear lamina while the LINC complex remains intact.
Nucleus, 2014, 5:25 -24

Graciotti, M.; Alam, M.; Solyakov, L.; Schmid, R.; Burley, G.; Bottrill, A.R.; Doerig, C.; Cullis, P.; Tobin, A.B.
Malaria Protein Kinase CK2 (PfCK2) Shows Novel Mechanisms of Regulation.
PLOS ONE, 2014, 9, e85391. DOI: 10.1371/journal.pone.0085391

Dorin-Semblat, D.; Bottrill, A.R.; Solyakov, L.; Tobin, A.B.; Doerig, C.
Experimental Tools for the Study of Protein Phosphorylation in Plasmodium.
Methods in Molecular Biology, 2013, 923, 241-257

Moiseeva, T.N.; Bottrill, A.R.; Melino, G.; Barlev, N.A.
DNA damage-induced ubiquitylation of proteasome controls its proteolytic activity.
Oncotarget, 2013, 4, 1338-1348.

Zaykova, I.I.; Kulichkova, V.A.; Ermolaeva, I.B.; Bottrill, A.R.; Barlev, N.A.; Tsimokha, A.S.
Characterization of the extra-cellular Proteasomes and its interacting Proteins by iTRAQ mass spectrometry.
Cell and Tissue Biology, 2013, 7, 253-265
(Original article: Tsitologiya, 2013, 55, 111-122)

Roberts, J.A.; Bottrill, A.R.; Mistry, S.; Evans, R.J.
Mass spectrometry analysis of human P2X1 receptors; insight into phosphorylation, modelling and conformational changes.
Journal of Neurochemistry, 2012, 123, 725-735

Schuurmans Stekhoven, F.M.A.H.; Lee, T.-H.; Wang, P.-J.; Jang-Liaw, N.-H.; Tanaka, S.; Bottrill, A.R.
Proteomic Studies on various organs and tissues of the Frilled Shark Chlamydoselachus anguineus.
Zoological Studies, 2012, 51, 1248-1269

Makarov, E.; Owen, N.; Bottrill, A.R.; Makarova, O.
Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs.
Nucleic Acids Research, 2012, 40, 2639-2652

Solyakov, L.; Halbert, J.; Alam, M.M.; Semblat, J.-P.; Dorin-Semblat, D.; Reininger, L.; Bottrill, A.R.; Mistry, S.C.; Abdi, A.; Fennell, C.; Holland, Z.; Demarta, C.; Bouza, Y.; Sicard, A.; Nivez, M.-P.; Eschenlauer, S.; Lama, T.; Thomas. D.C.; Sharma, P.; Agarwal, S.; Kern, S.; Pradel, G.; Graciotti, M.; Tobin, A.B.; Doerig, C.
Global kinomic and phospho-proteomic analyses of the human malaria parasite Plasmodium falciparum
Nature Communications, 2:565|DOI: 10.1038/ncomms1558

Fedorova, O.A.; Moiseeva, T.N.; Nikiforov, A.A.; Tsimokha, A.S.; Livinskaya, V.A.; Hodgson, M.; Bottrill, A.R.; Evteeva, I.N.; Ermolayeva, J.B.; Kuznetzova, I.M.; Turoverov, K.K.; Eperon, I.; Barlev, N.A.
Proteomic analysis of the 20S proteasome (PSMA3)-interacting proteins reveals a functional link between the proteasome and mRNA metabolism.
Biochemical and Biophysical Research Communications, 2011, 416, 258-265

Butcher, A.; Prihandoko, R.; Kong, K.C.; McWilliams, P.; Edwards, J.M.; Bottrill, A.R.; Mistry, S.C.; Tobin, A.B.
Differential G-protein coupled receptor phosphorylation provides evidence for a signalling barcode.
Journal of Biological Chemistry, 2011, 286, 11506-11518

Bottrill, A.R.; Leckie, B.
A specific receptor for the prorenin propart peptide Arg10-Arg20 does not occur on human endothelial cells.
Journal of the Renin-Angiotensin-Aldosterone System, 2011, 12, 36-41

Poulin, B.; Butcher, A.; McWilliams, P.; Bourgognon, J.-M.; Pawlak, R.; Kong, K.C.; Bottrill, A.R.; Mistry, S.C.; Wess, J.; Rosethorne, E.M.; Charlton, S.J.; Tobin, A.B.
The M3-muscarinic receptor regulates learning and memory in a receptor-phosphorylation/arrestin dependent manner.
PNAS, 2010, 107, 9440-9445.

Wendelaar Bonga, S.E.; Lee, T.-H.; Bottrill, A.R.; Schuurmanns Stekhoven, F.M.A.H.
Presence or absence of the Cl­ˉ channel phospholemman in the rectal gland of sharks: a comparative study.
Zoological Studies, 2010, 49, 326-334.

Kulichkova, V.A.; Tsimokha, A.S.; Fedorova, O.A.; Moiseeva, T.N.; Bottrill, A.R.; Lezina, L.; Gauze, L.N.; Konstantinova, I.M.; Mittenberg, A.G.; Barlev, N.A.
26S proteasome exhibits endoribonuclease activity controlled by extra-cellular stimuli.
Cell Cycle, 2010, 9, 840-849.

Bella, A.; Lewis, H.; Phu, J.; Bottrill, A.R.; Mistry, S.C.; Pullar, C.E.; Ryadnov, M.G.
Conformationally constrained mimetics of laminin peptide YIGSR as precursors for antimetastatic disintegrins.
Journal of Medicinal Chemistry, 2009, 52, 7966-7969.

Butcher, A.J.; Torrecilla, I.; Young K.W.; Kong, K.C.; Mistry, S.C.; Bottrill, A.R.; Tobin, A.B.
NMDA-receptors mediate the phosphorylation and desensitisation of muscarinic receptors in cerebellar granule neurones.
Journal of Biological Chemistry, 2009, 284, 17147-17156.

Pipirou, Z.; Guallar, V.; Basran, J.; Metcalfe, C.L.; Murphy, E.J.; Bottrill, A.R.; Mistry, S.C.; Raven, E.L.
Peroxide-dependent Formation of a Covalent Link between Trp51 and the Heme in Cytochrome c Peroxidase.
Biochemistry, 2009, 48, 3593-3599.

Turapov, O.A.; Mukamolova, G.V.; Basran, J.; Bottrill, A.R.; Pangburn, M.K.
Digestion of Native proteins for Proteomics using a Thermocycle.
Analytical Chemistry, 2008, 80, 6093-6099.

Pipirou, Z.; Bottrill, A.R.; Svistunenko, D.; Efimov, I; Basran, J.; Mistry, S.C.; Cooper, C.; Raven, E.L.
The Reactivity of Heme in Biological Systems: Autocatalytic formation of both Tyrosine-heme and Tryptophan-heme covalent links in a single protein architecture.
Biochemistry, 2007, 46, 2174-2180.

Nuhse, T.S.; Bottrill, A.R.; Jones, A.; Peck, S.C.
Quantitative Phosphoproteomic Analysis of Plasma Membrane Proteins reveals Regulatory Mechanisms of Plant Innate Immune Responses.
Plant Journal, 2007, 51, 931-940.

Pipirou, Z.; Bottrill, A.R.; Metcalfe, C.M.; Mistry, S.C.; Badyal, S.K.; Rawlings, B.J.; Raven, E.L.
Autocatalytic formation of a covalent link between Trp41 and heme in ascorbate peroxidase.
Biochemistry, 2007, 46, 2174-2180.

Parks, W.M.; Bottrill, A.R.; Pierrat, O.A.; Durrant, M.C.; Maxwell, A.
The action of the bacterial toxin, microcin B17, on DNA gyrase.
Biochemie, 2007, 89, 500-507.

Widdick, D.A.; Dilks, K.; Chandra, G.; Bottrill, A.R.; Naldrett, M.J.; Pohlschroder, M.; Palmer, T.
The twin arginine translocation pathway is a major route of protein export in Streptomyces coelicolor.
PNAS, 2006, 47, 17927-17932.

Bayer, E.M.; Bottrill, A.R.; Walshaw, J.; Vigouroux, M.; Naldrett, Thomas, C.L.; Maule, A.J.
Arabidopsis cell wall proteome defined using multidimensional protein identification technology.
Proteomics, 2006, 6, 301-311.

Escutia, M.R.; Bowater, L.; Edwards, A.; Bottrill, A.R.; Burrell, M.; Polanco, R.; Vicuna, R.; Bornemann, S.
Cloning and sequencing of two Ceriporiopsis subvermispora bicupin oxalate oxidase allelic isoforms: implications for the reaction specificity of oxalate oxidases and decarboxylases.
Applied and Environmental Microbiology, 2005, 71, 3608-3616.

Moreno, F.J.; Mellon, F.A.; Martin, M.S.J.; Bottrill, A.R.; Mills, E.N.C.
Stability of the major allergen Brazil nut 2S albumin (Ber e 1) to physiologically relevant in vitro gastrointestinal digestion.
FEBS Journal, 2005, 272, 341-352.

Bashir, S.; Bottrill, A.R.; Padley, H.D.; Critchley, P.; Giannakopulos A.E.
Matrix-assisted Laser Desorption/ionisation Mass Spectrometry. A comparison of Fragmentation Patterns of Linear Dextran Obtained by In-Source Decay, Post-Source Decay and Collision-induced-dissociation and the Stability of Linear and Cyclic Glycans Studied by In-Source Decay.
European Journal of Mass Spectrometry, 2004, 1, 109-120.

Li, W.; Bottrill, A.R.; Bibb, M.J.; Buttner, M.J.; Paget, M.S.B.; Kleanthous, C.
The Role of Zinc in the Disulphide Stress-Regulated Anti-Sigma Factor RsrA from Streptomyces coelicolor.
Journal of Molecular Biology, 2003, 333, 461-472.

Johnson, P.E.; Patron, N.J.; Bottrill, A.R.; Dinges,J.R.; Fahy, B.F.; Parker, M.L.; Waite, D.N.; Denyer, K.
A Low-Starch Barley Mutant, Risø 16 Lacking the Cytosolic Small Subunit of ADP-Glucose Pyrophosphorylase, Reveals the Importance of the Cytosolic Isoform and the Identity of the Plastidial Small Subunit.
Plant Physiology, 2003, 131, 684-696.

Chan,J.; Mao,G.; Smertenko, A.; Hussey, P.; Naldrett, M.J.; Bottrill, A.R.; Lloyd, C.
Identification of a MAP65 Isoform Involved in Directional Expansion of Plant Cells.
FEBS Letters, 2003, 534, 161-163.

Bottrill, A.R.; Giannakopulos, A.E.; Millichope, A.; Lee, K.S.; Derrick, P.J.
Combinations of time-of-flight mass analysers with magnetic-sector instruments: in-line and perpendicular arrangements. Applications to poly(ethylene glycol) with long-chain end groups.
European Journal of Mass Spectrometry, 2000, 6, 225-232.

Bottrill, A.R.; Giannakopulos, A.E.; Waterson, C.; Haddleton, D.M.; Lee, K.S.; Derrick, P.J.
Determination of end groups of synthetic polymers by matrix-assisted laser desorption/ionization: High-energy collision-induced dissociation.
Analytical Chemistry, 1999, 71, 3637-3641.


Additional publications:

In addition to the authored publications listed above, my work has been incorporated into more than 60 publications documented by personal Acknowledgement:

Melo, J., et al., Listeria monocytogenes dairy isolates show a different proteome response to sequential exposure to gastric and intestinal fluids. International Journal of Food Microbiology, 2013. doi: 10.1016/j.ijfoodmicro.2013.03.001

Watkins, R.J., et al., A novel interaction between FRMD7 and CASK: evidence for a causal role in idiopathic infantile nystagmus. Human Molecular Genetics, 2013. doi: 10.1093/hmg/ddt060

Melo, J., et al., Proteomic Analysis Shows That Individual Listeria monocytogenes Strains Use Different Strategies in Response to Gastric Stress. Foodbourne Pathogens and Disease, 2013. 10(2): p. 107-119

Funaya, C., et al., Transient Structure Associated with the Spindle Pole Body Directs Meiotic Microtubule Reorganization in S. pombe. Current Biology, 2012. 22(7): p. 562-574

Melo, J., et al., Different assembly of acid and salt tolerance response in two dairy Listeria monocytogenes wild strains. Archives of Microbiology, 2012. doi: 10.1007/s00203-013-0878-6

Chauhan, N., et al., How is the distal pocket of a heme protein optimized for binding of tryptophan? FEBS Journal, 2012. 279(24): p. 4501-4509

Chen, Y.-J., et al., Identification of phosphorylation sites in the COOH-terminal tail of the μ-opioid receptor. Journal of Neurochemistry, 2012. DOI: 10.1111/jnc.12071

Lalo, U., et al., Heat Shock Protein 90 Inhibitors Reduce Trafficking of ATP-gated P2X1 Receptors and Human Platelet Responsiveness. Journal of Biological Chemistry, 2012. 287(39): p. 32747-32754.

Lalo, U., et al., Identification of human P2X1 receptor interacting proteins reveals a role of the cytoskeleton in receptor regulation. Journal of Biological Chemistry, 2011. doi: 10.1074/jbc.M111.253153.

Badyal, S.K., et al., Mechanism of the Ca2+-Dependent Interaction between S100A4 and Tail Fragments of Nonmuscle Myosin Heavy Chain IIA. Journal of Molecular Biology, 2011. 405(4): p. 1004-1026.

Johnson, M., et al., Fur is required for the activation of virulence gene expression through the induction of the sae regulatory system in Staphylococcus aureus. International Journal of Medical Microbiology, 2011. 301(1): p. 44-52

Moiseeva, T.N., et al., Effect of ubiquitination on peptidase activities of proteasomes in genotoxic stress. Doklady Biochemistry and Biophysics, 2010. 435(1): p. 307-311

Terra, V., et al., Characterization of novel beta-galactosidase activity that contributes to glycoprotein degradation and virulence in Streptococcus pneumoniae. Infection and Immunity, 2010. 78(1): p. 348-357

Su, W., et al., Highly Efficient Synthesis of DNA-Binding Hairpin Polyamides via the Use of a New Triphosgene Coupling Strategy. Organic Letters, 2009. 11(17): p. 3910-3913

Badyal, S.K., et al., Evidence for Heme Oxygenase Activity in a Heme Peroxidase. Biochemistry, 2009. 48(22): p. 4738-4746

Bush, M.S., et al., Selective recruitment of proteins to 5' cap complexes during the growth cycle in Arabidopsis. Plant Journal, 2009. 59(3): p. 400-412

Itoh, T., et al., Structural Basis for the Activation of PPARg by Oxidized Fatty Acids. Nature Structural & Molecular Biology., 2008. 15(9): p. 924-931.

Monk, C.E., et al., Oxygen- and NssR-dependent Globin Expression and Enhanced Iron Acquisition in the Response of Campylobacter to Nitrosative Stress. Journal of Biological Chemistry, 2008. 283(42): p. 28413-28425.

Basran, J., et al., A Kinetic, Spectroscopic, and Redox Study of Human Tryptophan 2,3-Dioxygenase. Biochemistry, 2008. 47(16): p. 4752-4760.

Merkouropoulos, G., et al., An Arabidopsis Protein Phosphorylated in Response to Microbial Elicitation, AtPHOS32, is a Substrate of MAP Kinases 3 and 6. J. Biol. Chem., 2008. 283(16): p. 10493-10499.

Johnson, M., et al., Iron-Regulated Biofilm Formation in Staphylococcus aureus Newman Requires ica and the Secreted Protein Emp. Infection and Immunity, 2008. 76: p. 1756-1765.

Pappa, E.C., et al., Application of Proteomic techniques to Protein and Peptide Profiling of Teleme Cheese made from different types of milk. Int. Dairy J., 2008. 18: p. 605-614.

Koroleva, O., et al., Proteomic Analysis of the Plant Nucleolus. Plant Proteomics (Book), 2007: p 247-269. DOI: 10.1007/978-3-540-72617-3_16

Polley, A.C.J., et al., Proteomic Analysis Reveals Field-Wide Changes in Protein Expression in the Morphologically Normal Mucosa of Patients with Colorectal Neoplasia. Cancer Res., 2006. 66(13): p. 6553-6562.

Durand, A. and M. Merrick, In Vitro Analysis of the Escherichia coli AmtB-GlnK Complex Reveals a Stoichiometric Interaction and Sensitivity to ATP and 2-Oxoglutarate. J. Biol. Chem., 2006. 281(40): p. 29558-29567.

del Rocio Leon-Kempis, M., et al., The Campylobacter jejuni PEB1a adhesin is an aspartate/glutamate-binding protein of an ABC transporter essential for microaerobic growth on dicarboxylic amino acids. Molecular Microbiology, 2006. 60(5): p. 1262-1275.

Badyal, S.K., et al., Conformational mobility in the active site of a heme peroxidase. J. Biol. Chem., 2006. 281(34): p. 24512-24520.

Stevenson, C.E.M., et al., Crystallization and Preliminary X-ray analysis of the RAD protein from Antirrhinum majus. Acta Cryst. F, 2005. 61(10): p. 885-888.

Yoshida, S. and M. Parniske, Regulation of Plant Symbiosis Receptor Kinase through Serine and Threonine Phosphorylation. J. Biol. Chem., 2005. 280(10): p. 9203-9209.

van der Hoorn, R.A.L., et al., Structure-Function Analysis of Cf-9, a Receptor-Like Protein with Extracytoplasmic Leucine-Rich Repeats. Plant Cell, 2005: p. tpc.104.028118.

Tassoni, A., et al., Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures. New Phytologist, 2005. 166(3): p. 895-905.

Salt, L.J., et al., The identification of foam-forming soluble proteins from wheat (Triticum aestivum) dough. Proteomics, 2005. 5(6): p. 1612-1623.

Mohedano, M.L., et al., Evidence that the Essential Response Regulator YycF in Streptococcus pneumoniae Modulates Expression of Fatty Acid Biosynthesis Genes and Alters Membrane Composition. J. Bacteriol., 2005. 187(7): p. 2357-2367.

Korolev, A.V., et al., Identification of a novel family of 70 kDa microtubule-associated proteins in Arabidopsis cells. Plant J, 2005. 42(4): p. 547-555.

Kim, D.-W., et al., Effects of growth phase and the developmentally significant bldA-specified tRNA on the membrane-associated proteome of Streptomyces coelicolor. Microbiology, 2005. 151(8): p. 2707-2720.

Kim, D.-W., et al., Changes in the Extracellular Proteome Caused by the Absence of the bldA Gene Product, a Developmentally Significant tRNA, Reveal a New Target for the Pleiotropic Regulator AdpA in Streptomyces coelicolor. J. Bacteriol., 2005. 187(9): p. 2957-2966.

Holmes, K., et al., Campylobacter jejuni gene expression in response to iron limitation and the role of Fur Microbiology, 2005. 151(1): p. 243-257.

Harris, R.L., et al., Heterologous metalloprotein biosynthesis in Escherichia coli: conditions for the overproduction of functional copper- containing nitrite reductase and azurin from Alcaligenes xylosoxidans. Journal of Synchrotron Radiation, 2005. 12: p. 13-18.

D'Autreaux, B., et al., A non-haem iron centre in the transcription factor NorR senses nitric oxide. Nature, 2005. 437(29): p. 769-772.

Baker-Austin, C., et al., Molecular insight into extreme copper resistance in the extremophilic archaeon 'Ferroplasma acidarmanus' Fer1. Microbiology, 2005. 151(8): p. 2637-2646.

Andersen, M.T., et al., Diverse roles for HspR in Campylobacter jejuni revealed by the proteome, transcriptome and phenotypic characterization of an hspR mutant Microbiology, 2005. 151(3): p. 905-915.

Clemente, A., et al., Investigation of legume seed protease inhibitors as potential anit-carcinogenic proteins. Recent Advances of Research in Antinutritional Factors in Legume Seeds and Oilseeds, 2004. 110: p. 137-141.

Bowater, L., et al., Bacillus subtilis YxaG is a novel Fe-containing quercetin 2,3-dioxygenase FEBS. Letters, 2004. 557: p. 45-48.

Hutchins, A.P., et al., In vivo interaction between CDKA and eIF4A: a possible mechanism linking translation and cell proliferation. FEBS Letters, 2004. 556(1-3): p. 91-94.

Wilmes, P. and P.L. Bond, The application of two-dimensional polyacrylamide gel electrophoresis and downstream analyses to a mixed community of prokaryotic microorganisms. Environmental Microbiology, 2004. 6(9): p. 911-920.

van der Hoorn, R.A.L., et al., Activity Profiling of Papain-Like Cysteine Proteases in Plants. Plant Physiol., 2004. 135(3): p. 1170-1178.

Patron, N.J., et al., The lys5 Mutations of Barley Reveal the Nature and Importance of Plastidial ADP-Glc Transporters for Starch Synthesis in Cereal Endosperm. Plant Physiol., 2004. 135(4): p. 2088-2097.

Niggeweg, R., A.J. Michael, and C. Martin, Engineering plants with increased levels of the antioxidant chlorogenic acid. Nature Biotechnology, 2004. 22(6): p. 746-754.

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Forthcoming and Recent Lectures, Events and Training

12th Proteomics Methods Forum - Cardiff - Tuesday 23rd June 2015 - Chair of Post-translational Modifications Session.

13th East Midlands Proteomics Workshop - Wednesday 19th November 2014 - Chair of Meeting, welcoming address and host for first session.

Midlands Integrative Biosciences Training Partnership - Proteomics Masterclass - 17/19/20 November 2014