Publications

Advance Imaging Facility

If you publish work supported by the AIF, e.g. by using one or more of our microscopes or support in image or data analysis, it is obligatory to include a sentence in the acknowledgement in addition to any personal acknowledgement. For example:

We thank the Advanced Imaging Facility at the University of Leicester for support.

To keep this list up-to-date, please email with any appropriate publications obtained using the Advanced Imaging Facility.

2019

Al-Ismaeel Q , Neal CP, Al-Mahmoodi H, Almutairi Z, Al-Shamarti I, Straatman K, Jaunbocus N, Irvine A, Issa E, Moreman C, Dennison AR, Sayan AE, McDearmid J, Greaves P, Tulchinsky E,  Kriajevska M (2019) ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins. British Journal of Cancer https://doi.org/10.1038/s41416-019-0483-9

Ekpenyong-Akiba AE, Canfarotta F, Bashar Abd H, Poblocka M, Casulleras M, Castilla-Vallmanya L, Kocsis-Fodor G, Kelly ME, Janus J, Althubiti M, Piletska E, Piletskyc S, Macip S (2019) Detecting and targeting senescent cells using molecularly imprinted nanoparticles. Nanoscale Horiz. 4: 757-768. DOI: 10.1039/c8nh00473k.

Repici M, Hassanjani M, Maddison DC, Garção P, Cimini S, Patel B, Szegö EM, Straatman KR, Lilley KS, Borsello T, Outeiro TF, Panman L, Giorgini F (2019) The Parkinson's disease-linked protein DJ-1 associates with cytoplasmic mRNP granules during stress and neurodegeneration. Mol Neurobiol 56:61-77. doi: 10.1007/s12035-018-1084-y. PubMed.

O’Regan L, Barone G, Adib R, Woo CG, Jeong HJ, Richardson EL, Richards MW, Muller PAJ, Collis SJ, Fennell DA, Choi J, Bayliss R, Fry AM (2019) EML4-ALK V3 drives cell migration through NEK9 and NEK7 kinases in non-small-cell lung cancer. bioRxiv . doi.org/10.1101/567305

2018

Hey F, Andreadi C, Noble C, Patel B, Jin H, Kamata T, Straatman K, Luo J, Balmanno K, Jones DTW, Collins VP, Cook SJ, Caunt CJ, Pritchard C (2018) Over-expressed, N-terminally truncated BRAF is detected in the nucleus of cells with nuclear phosphorylated MEK and ERK. Heliyon. 4:e01065. PubMed.

Fry AMAdib RMontgomery JMAtherton JO'Regan L, Richards MStraatman KRRoth DStraube ABayliss RMoores CA (2018) Mitotic phosphorylation by Nek6 and Nek7 reduces microtubule affinity of EML4 to alter spindle dynamics and promote chromosome congression.

Bird MF, Guerrini R, Willets JM, Thompson JP, Caló G, Lambert DG (2018) Nociceptin/Orphanin FQ (N/OFQ) conjugated to ATTO594; a novel fluorescent probe for the NOP receptor. Br J Pharmacol.  doi: 10.1111/bph.14504. PubMed.

Chung WY, Wanford JJ, Kumar R, Isherwood JD, Haigh RD, Oggioni MR, Dennison AR, Ercoli G (2018) An ex vivo porcine spleen perfusion as a model of bacterial sepsis. ALTEX. 2018 Aug 3. doi: 10.14573/altex.1805131. PubMed.

Jones PD, Kaiser MA, Ghaderi Najafabadi M, Koplev S, Zhao Y, Douglas G, Kyriakou T, Andrews S, Rajmohan R, Watkins H, Channon KM, Ye S, Yang X, Björkegren JLM, Samani NJ, Webb TR (2018) JCAD, a Gene at the 10p11 Coronary Artery Disease Locus, Regulates Hippo Signaling in Endothelial Cells. Arterioscler Thromb Vasc Biol. 38:1711-1722. PubMed.

Al-Osta I, Mucha M, Pereda D, Piqué-Gili M, Okorocha AE, Thomas R, Hartell NA (2018) Imaging calcium in hippocampal presynaptic terminals with a ratiometric calcium sensor in a novel transgenic mouse. Front Cell Neurosci. 12:209. doi: 10.3389/fncel.2018.00209. PubMed.

Kelsall EJ, Vertesy AStraatman KTariq MGadea RParmar CSchreiber GRandhawa SDominguez CKlipp ETanaka K (2018) Constitutively active RAS in S. pombe causes persistent Cdc42 signalling but only transient MAPK activation.

Ercoli G , Fernandes VE, Chung WY, Wanford JJ, Thomson S, Christopher D. Bayliss, Straatman K, Crocker PR, Dennison A, Martinez-Pomares L, Andrew PW, Moxon ER, Oggioni MR (2018) Intracellular replication of Streptococcus pneumoniae inside splenic macrophages serves as a reservoir for septicaemia. Nature Microbiology: doi:10.1038/s41564-018-0147-1. PubMed

2017

Fry AM, Bayliss R, Roig J (2017) Mitotic Regulation by NEK Kinase Networks.Front Cell Dev Biol. 1: 102. doi: 10.3389/fcell.2017.00102. PubMed.

Elgamoudi BA, Ketley JM (2017) Lighting up my life: a LOV-based fluorescent reporter for Campylobacter jejuni. Res Microbiol. pii: S0923-2508(17)30169-9. PubMed.

Rada M, Althubiti M, Ekpenyong-Akiba AE, Lee KG, Lam KP, Fedorova O, Barlev NA, Macip S (2017) BTK blocks the inhibitory effects of MDM2 on p53 activity.Oncotarget. 8:106639-106647. PubMed.

Sampson J, O'Regan L, Dyer MJS, Bayliss R, Fry AM (2017) Hsp72 and Nek6 Cooperate to Cluster Amplified Centrosomes in Cancer Cells.Cancer Res. 77:4785-4796. PubMed.

Kamata T, Giblett S, Pritchard C (2017) KRASG12D expression in lung-resident myeloid cells promotes pulmonary LCH-like neoplasm sensitive to statin treatment. Blood. doi: 10.1182/blood-2017-02-770149. PubMed.

Rada M, Vasileva E, Lezina L, Marouco D, Antonov AV, Macip S, Melino G, Barlev NA (2017) Human EHMT2/G9a activates p53 through methylation-independent mechanism. Oncogene.36:922-932. PubMed.

Sepsi A, Higgins JD, Heslop-Harrison JS, Schwarzacher T (2017) CENH3 morphogenesis reveals dynamic centromere associations during synaptonemal complex formation and the progression through male meiosis in hexaploid wheat. Plant J. 89: 235-249. PubMed.

2016

Samuel J, Jayne S, Chen Y, Majid A, Wignall A, Wormull T, Najeeb H, Luo JL, Jones GDD, Macip S, Dyer MJS (2016) Posttranscriptional Upregulation of p53 by Reactive Oxygen Species in Chronic Lymphocytic Leukemia. Cancer Res 76: 6311 - 6319. PubMed.

Althubiti M, Rada M, Samuel J, Escorsa JM, Najeeb H, Lee KG, Lam KP, Jones GD, Barlev NA, Macip S (2016) BTK Modulates p53 Activity to Enhance Apoptotic and Senescent Responses. Cancer Res. 76:5405 - 5414. PubMed.

Banushi B, Forneris F, Straatman-Iwanowska A, Strange A, Lyne AM, Rogerson C, Burden JJ, Heywood WE, Hanley J, Straatman KR, Smith H, Bem D,  Kriston-Vizi J, Ariceta G, Risteli M, Wang C, Waddington SN, Howe SJ, Ferraro F, Gjinovci A, Lawrence S, Marsh M, Girolami M, Bozec L, Mills K, Gissen P (2016) Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nature Communications. PubMed.

Jones PD, Kaiser MA, Ghaderi Najafabadi M, McVey DG, Beveridge AJ, Schofield CL, Samani NJ, Webb TR (2016) The coronary artery disease associated coding variant in zinc finger C3HC-type containing 1 (ZC3HC1) affects cell cycle regulation. J Biol Chem. 291: 16318-16327. PubMed.

2015

Haq T, Richards MW, Burgess SG, Gallego P, Yeoh S, O'Regan L, Reverter D, Roig J, Fry AM, Bayliss R (2015) Mechanistic basis of Nek7 activation through Nek9 binding and induced dimerization. Nat Commun. 6:8771. doi: 10.1038/ncomms9771. PubMed.

Sabir SR, Sahota NK, Jones GD, Fry AM (2015) Loss of Nek11 Prevents G2/M Arrest and Promotes Cell Death in HCT116 Colorectal Cancer Cells Exposed to Therapeutic DNA Damaging Agents. PLOS ONE 10:e0140975. doi: 10.1371. PubMed.

Roach KM, Feghali-Bostwick CA, Amrani Y, Bradding P (2015) Lipoxin A4 Attenuates Constitutive and TGF-β1–Dependent Profibrotic Activity in Human Lung Myofibroblasts. J Immunol. 195: 2852–2860. PubMed.

Kamata T, Jin H, Giblett S, Patel F, Patel F, Foster C, Pritchard C (2015) The cholesterol-binding protein NPC2 restrains recruitment of stromal macrophage-lineage cells to early-stage lung tumours. EMBO Mol Med. 2015 Jul 16. pii: e201404838. doi: 10.15252/emmm.201404838. PubMed.

Prosser SL, Sahota NK, Pelletier L, Morrison CG, Fry AM (2015) Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis. J Cell Biol. 209:339-348. PubMed.

O'Regan L, Sampson J, Richards MW, Knebel A, Roth D, Hood FE, Straube A, Royle SJ, Bayliss R, Fry AM (2015) Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression. J Cell Biol. 209: 349-358. PubMed.

Richards MW, O'Regan L, Roth D, Montgomery JM, Straube A, Fry AM, Bayliss R (2015) Microtubule association of EML proteins and the EML4-ALK variant 3 oncoprotein require an N-terminal trimerization domain. Biochem J. 467: 529-536. PubMed.

Jay M, De Faveri F, McDearmid JR (2015) Firing dynamics and modulatory actions of supraspinal dopaminergic neurons during zebrafish locomotor behaviour. Curr Biol. 25:435-44. PubMed.

Breda C, Nugent ML, Estranero JG, Kyriacou CP, Outeiro TF, Steinert JR, Giorgini F (2015) Rab11 modulates α-synuclein mediated defects in synaptic transmission and behaviour. Hum Mol Genet 24: 1077-1091. PubMed.

2014

Roach KM, Wulff H, Feghali-Bostwick C, Amrani Y, Bradding P (2014)  Increased constitutive αSMA and Smad2/3 expression in idiopathic pulmonary fibrosis myofibroblasts is KCa3.1-dependent. Respir Res. 15: 155. PubMed.

Dissel S, Hansen CN, Ozkaya O, Hemsley M, Kyriacou CP, Rosato E (2014) The logic of circadian organization in Drosophila. Curr Biol. 24: 2257-2266. doi: 10.1016/j.cub.2014.08.023. PubMed.

Althubiti M, Lezina L, Carrera S, Jukes-Jones R, Giblett SM, Antonov A, Barlev N, Saldanha GS, Pritchard CA, Cain K, Macip S (2014) Characterization of novel markers of senescence and their prognostic potential in cancer. Cell Death Dis. 20:e1528. PubMed.

Patel JT, Bottrill A, Prosser SL, Jayaraman S, Straatman K, Fry AM, Shackleton S (2014) Mitotic phosphorylation of SUN1 loosens its connection with the nuclear lamina while the LINC complex remains intact. Nucleus 5: 462 - 473. PubMed.

Meinke P, Mattioli E, Haque F, Antoku S, Columbaro M, Straatman KR, Worman HJ, Gundersen GG, Lattanzi G, Wehnert M and Shackleton S (2014) Muscular Dystrophy-Associated SUN1 and SUN2 Variants Disrupt Nuclear-Cytoskeletal Connections and Myonuclear Organization. PLoS Genet. 10(9):e1004605. PubMed.

Zondler L, Miller-Fleming L, Repici M, Gonçalves S, Tenreiro S, Rosado-Ramos R, Betzer C, Straatman KR, Jensen PH, Giorgini F, Outeiro TF (2014) DJ-1 interactions with α-synuclein attenuate aggregation and cellular toxicity in models of Parkinson's disease. Cell Death Dis. 5, e1350; doi:10.1038/cddis.2014.307. PubMed.

Jamaladdin S, Kelly RD, O'Regan L, Dovey OM, Hodson GE, Millard CJ, Portolano N, Fry AM, Schwabe JW, Cowley SM (2014) Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc. Natl. Acad. Sci. USA. 111: 9840-9845. PubMed.

Hardy T, Lee M, Hames RS, Prosser SL, Cheary DM, Samant MD, Schultz F, Baxter JE, Rhee K, Fry AM (2014) Multisite phosphorylation of C-Nap1 releases it from Cep135 to trigger centrosome disjunction. J Cell Sci. 127: 2493-2506. PubMed.

Carrera S, Senra J, Acosta MI, Althubiti M, Hammond EM, de Verdier PJ, Macip S (2014) The role of the HIF-1α transcription factor in increased cell division at physiological oxygen tensions. PLoS One. 9:e97938. PubMed.

Moiseeva EP, Straatman KR, Leyland ML and Bradding P (2014) CADM1 Controls Actin Cytoskeleton Assembly and Regulates Extracellular Matrix Adhesion in Human Mast Cells. PLOS ONE 9: e85980. PubMed.

Ahmed SB, Prigent SA (2014) A nuclear export signal and oxidative stress regulate ShcD subcellular localisation: a potential role for ShcD in the nucleus. Cell Signal. 26:32-40. PubMed.

Jay M, Bradley S, McDearmid JR (2014) Effects of nitric oxide on neuromuscular properties of developing zebrafish embryos. PLoS One. 9(1):e86930. PubMed.

2013 

Dhani DK, Goult BT, George GM, Rogerson DT, Bitton DA, Miller CJ, Schwabe JW and Tanaka K (2013) Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3(Alp6). Mol Biol Cell. 24:3337-3349. PubMed.

Goult BT, Xu XP, Gingras AR, Swift M, Patel B, Bate N, Kopp PM, Barsukov IL, Critchley DR, Volkmann N, Hanein D (2013) Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: implications for talin activation.J Struct Biol. 184:21-32. PubMed.

Blakemore LM, Boes C, Cordell R and Manson MM (2013) Curcum in-induced mitotic arrest is characterized by spindle abnormalities, defects in chromosomal congression and DNA damage. Carcinogenesis. 34:351-60. PubMed

Cooke RM, Mistry R, Challiss RA and Straub VA (2013) Nitric oxide synthesis and cGMP production is important for neurite growth and synapse remodeling after axotomy. J Neurosci. 33: 5626-37. PubMed 

Carrera S, Cuadrado-Castano S, Samuel J, Jones GD, Villar E, Lee SW, Macip S (2013) Stra6, a retinoic acid-responsive gene, participates in p53-induced apoptosis after DNA damage. Cell Death Differ 20:910-9. PubMed.

Watkins RJ, Patil R, Goult BT, Thomas MG, Gottlob I, and Shackleton S (2013) A novel interaction between FRMD7 and CASK: evidence for a causal role in idiopathic infantile nystagmus. Hum Mol Genet. Feb 20. PubMed.

Varadarajan S, Tanaka K, Smalley JL, Bampton ET, Pellecchia M, Dinsdale D, Willars GB and Cohen GM (2013) Endoplasmic reticulum membrane reorganization is regulated by ionic homeostasis. PLoS One 8:e56603. PubMed.

Raschpichler M, Straatman K, Pampel A, Schroeter ML, Arelin K, Schloegl H, Fritzsch D, Mende M, Stumvoll M, Villringer A and Mueller K (2013) Abdominal fat distribution and its relationship to brain changes - the differential effects of age on cerebellar structure and function: a cross-sectional, exploratory study. BMJopen;3:e001915. PubMed.

Repici M, Straatman KR, Balduccio N, Enguita FJ, Outeiro TF and Giorgini F (2013) Parkinson’s disease-associated mutations in DJ-1 modulate its dimerization in living cells. J. Mol. Med. 91:599-611. PubMed.

Venoux M, Tait X, Hames RS, Straatman KR, Woodland HR and Fry AM (2013) Poc1A and Poc1B act together in human cells to ensure centriole integrity. J Cell Sci. 126: 163-175. PubMed

2012

Prosser SL, Samant MD, Baxter JE, Morrison CG and Fry AM (2012) Oscillation of APC/C activity during cell cycle arrest promotes centrosome amplification. J Cell Sci. 125: 5353-5368. PubMed.

Andreadi C, Cheung LK, Giblett S, Patel B, Jin H, Mercer K, Kamata T, Lee P, Williams A, McMahon M, Marais R and Pritchard C (2012) The intermediate-activity L597V-BRAF mutant acts as an epistatic modifier of oncogenic RAS by enhancing signaling through the RAF/MEK/ERK pathway. Genes Dev.; 26: 1945–1958. PubMed.

Funaya C, Samarasinghe S, Pruggnaller S, Ohta M, Connolly Y, Jan Müller J Murakami H, Grallert A, Yamamoto M, Smith D, Antony C and Tanaka K (2012) Transient Structure Associated with the Spindle Pole Body Directs Meiotic Microtubule Reorganization in S. pombe. Current Biology 22: 562-574. PubMed

Lalo U, Jones S, Roberts JA, Mahaut-Smith MP and Evans RJ (2012) Heat shock protein 90 inhibitors reduce trafficking of ATP-gated P2X1 receptors and human platelet responsiveness. J Biol Chem. 287: 32747-32754. PubMed.

Baines RJ, Chana RS, Hall M, Febbraio M, Kennedy D, Brunskill NJ (2012) CD36 mediates proximal tubular binding and uptake of albumin and is upregulated in proteinuric nephropathies. Am J Physiol Renal Physiol. 303:F1006-14. PubMed

Elliott PR, Irvine AF, Jung HS, Tozawa K, Pastok MW, Picone R, Badyal SK, Basran J, Rudland PS, Barraclough R, Lian L-Y, Bagshaw CR, Kriajevska M and Barsukov IL (2012) Asymmetric Mode of Ca2+-S100A4 Interaction with Nonmuscle Myosin IIA Generates Nanomolar Affinity Required for Filament Remodeling. Structure 20: 654-666. PubMed.

Masgras I, Carrera S, de Verdier PJ, Brennan P, Majid A, Makhtar W, Tulchinsky E, Jones GD, Roninson IB, Macip S (2012) Reactive oxygen species and mitochondrial sensitivity to oxidative stress determine induction of cancer cell death by p21. Biol Chem. 287:9845-54 PubMed.

Praekelt U, Kopp PM, Rehm K, Linder S, Bate N, Patel B, Debrand E, Manso AM, Ross RS, Conti F, Zhang MZ, Harris RC, Zent R, Critchley DR, Monkley SJ (2012) New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2. Eur J Cell Biol. 91: 180-191. PubMed.

Zalli D, Bayliss R, Fry AM (2012) The Nek8 protein kinase, mutated in the human cystic kidney disease nephronophthisis, is both activated and degraded during ciliogenesis. Hum Mol Genet. 21: 1155-1171. PubMed.

2011

Sylvius N, Bonne G, Straatman K, Reddy T, Gant TW and Shackleton S (2011) MicroRNA expression profiling in patients with lamin A/C associated muscular dystrophy. FASEB J. 25:3966-3978. PubMed.

Smith E, Hégarat N, Vesely C, Roseboom I, Larch C, Streicher H, Straatman K, Flynn H, Skehel M, Hirota T, Kuriyama R and Hochegger H (2011) Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1. EMBO J. 30: 2233-2245. PubMed.

van de Ven C, Bialecka M, Neijts R, Young T, Rowland JE, Stringer EJ, Van Rooijen C, Meijlink F, Nóvoa A, Freund JN, Mallo M, Beck F, Deschamps J (2011) Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone. Development. 138:3451-62. PubMed.

Thomas MG, Kumar A, Thompson JR, Proudlock FA, Straatman K, and Gottlob I (2011) Is high-resolution spectral domain optical coherence tomography reliable in nystagmus? Br J Ophthalmol 2011-300476. PubMed.  

Lopes CA, Prosser SL, Romio L, Hirst RA, O'Callaghan C, Woolf AS and Fry AM (2011) Centriolar satellites are assembly points for proteins implicated in human ciliopathies, including oral-facial-digital syndrome 1. J Cell Sci. 124:600-12. PubMed

Monkley SJ, Kostourou V, Spence L, Petrich B, Coleman S, Ginsberg MH, Pritchard CA and Critchley DR (2011) Endothelial cell talin1 is essential for embryonic angiogenesis. Dev Biol. 349:494-502. PubMed

Publications 2010 - 2005

Cruse G, Yang W, Duffy SM, Chachi L, Leyland M, Amrani Y and Bradding P (2010) Counterregulation of b2-adrenoceptor function in human mast cells by stem cell factor. J. Allergy Clin. Immunol. 125:257-263. PubMed.

Lalo U, Allsopp RC, Mahaut-Smith MP and Evans RJ (2010) P2X1 receptor mobility and trafficking; regulation by receptor insertion and activation. J. Neurochem. 113: 1177-1187. PubMed.

Bradley S, Tossell K, Lockley R and McDearmid JR (2010) Nitric Oxide Synthase Regulates Morphogenesis of Zebrafish Spinal Cord Motoneurons. J. Neurosci. 30: 16818 –16831. PubMed

Hayward DG, Newbatt Y, Pickard L, Byrne E, Mao G, Burns S, Sahota NK, Workman P, Collins I, Aherne W and Fry AM (2010) Identification by High-Throughput Screening of Viridin Analogs as Biochemical and Cell-Based Inhibitors of the Cell Cycle-Regulated Nek2 Kinase. J Biomol Screen. 15: 918-927. PubMed

Kopp PM, Bate N, Hansen TM, Brindle NPJ, Praekelt U, Debrand E, Coleman S, Mazzeo D, Goult BT, Gingras AR, Pritchard CA, Critchley DR and Monkley SJ (2010) Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1. Eur. J. Cell Biol. 89: 661-673. PubMed

Gingras A.R, Bate N, Goult BT, Patel B, Kopp PM, Emsley J, Barsukov IL, Roberts GC and Critchley DR (2010) The central region of talin has a unique fold that binds vinculin and actin. J Biol Chem. 285:29577-29587. PubMed.

Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM and Critchley DR (2010) Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1. J Cell Biochem. 109:808-817 . PubMed.

Haque F, Mazzeo D, Patel JT, Smallwood DT, Ellis JA, Shannahan CM and Shackleton S (2010) Mammalian SUN protein networks at the inner nuclear membrane and their role in laminopathy disease processes. J Biol Chem. 285: 3487-3498. PubMed.

Gehrig J, Reischl M,  Kalmar E,  Ferg M, Hadzhiev Y,  Zaucker A,  Song C, Schindler S, Liebel U and Müller F (2009) Automated high throughput mapping of promoter-enhancer interactions in zebrafish embryos. Nature Methods 6: 911 - 916. PubMed.

Sayan, A.E., Griffiths, T.R., Pal, R., Browne, G.B., Ruddick, A., Yagci, T., Edwards, R., Mayer, N.J., Qazi, H., Goyal, S., Fernandez, S., Straatman, K., Jones, G.D.D., Bowman, K.J., Colquhoun, A., Mellon, J.K., Kriajevska, M., and Tulchinsky, E. (2009) SIP1 protein protects cells from DNA damage-induced apoptosis and has independent prognostic value in bladder cancer. PNAS 106:14884-14889. PubMed.

O'Regan L. and Fry, A.M. (2009) The Nek6 and Nek7 Protein Kinases Are Required for Robust Mitotic Spindle Formation and Cytokinesis. Mol Cell Biol.;29:3975-3990. PubMed.

Debrand, E., El Jai, Y., Spence, L., Bate, N., Praekelt, U., Pritchard, C.A., Monkley, S.J. and Critchley, D.R. (2009) Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms. FEBS J. 276:1610-1628. PubMed.

Prosser, S.L. and Fry, A.M. (2009) Fluorescence imaging of the centrosome cycle in mammalian cells. Methods in Molecular Biology 545: 165-183. PubMed

Prosser, S.L., Straatman, K.R. and Fry, A.M. (2009) Molecular dissection of the centrosome overduplication pathway in S-phase arrested cells. Mol. Cell. Biol. 29: 1760-1773. PubMed.

Westwood, I., Cheary, D-M., Baxter, J.E., Richards, M.W., Fry, A.M. and Bayliss, R. (2009) Insights into the conformational variability and regulation of human Nek2 kinase. Journal of Molecular Biology 386: 476-485. PubMed.

Hames, R.S., Hames, R., Prosser, S.L., Euteneuer, U., Lopes, C.A.M., Moore, W., Woodland, H. and Fry, A.M. (2008) Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells. Experimental Cell Research 314: 574-589. PubMed.

Kimata, Y., Baxter, J.E., Fry, A.M. and Yamano, H. (2008) The Fizzy/Cdc20 family of proteins have a distinct role in the activation of the APC/C besides substrate recruitment. Molecular Cell 32: 576-583. PubMed.

Noble, C., Mercer, K., Hussain, J., Carragher, L., Giblett, S., Hayward, R., Patterson, C., Marais, R. and Pritchard, C.A. (2008) CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation. Molecular Cell 31: 862-872. PubMed.

Pattrick, M., Luckett, J., Yue, L. and Stover, C. (2008) Dual role of complement in adipose tissue. Mol Immunol. october. PubMed.

Stover, C.M., Luckett, J.C., Echtenacher, B., Dupont, A., Figgitt, S.E., Brown, J., Männel, D.N. and Schwaeble, W.J. (2008) Properdin plays a protective role in polymicrobial septic peritonitis. J Immunol. 180:3313-3318. PubMed.

Wickenden, J.A., Jin, H., Johnson, M., Gillings, A.S., Newson, C., Austin, M., Chell, S.D., Balmanno, K., Pritchard, C.A. and Cook S.J. (2008) Colorectal cancer cells with the BRAF(V600E) mutation are addicted to the ERK1/2 pathway for growth factor-independent survival and repression of BIM. Oncogene 27: 7150-7161. PubMed.

Codd V., D. Dolezel, J. Stehlik, A. Piccin, K.J. Garner, S.N. Racey, K.R. Straatman, E.J. Louis, R. Costa, I. Sauman, C.P. Kyriacou, E. Rosato (2007) Circadian Rhythm Gene Regulation in the Housefly Musca domestica. Genetics 177(3): 1539-5. PubMed.

Rellos, P., Ivins, F.J., Baxter, J.E., Pike, A., Nott, T.J., Parkinson, D-M., Das, S., Howell, S., Federov, O., Shen, Q.Y., Fry, A.M., Knapp, S. and Smerdon, S.J. (2007) Structure and regulation of the human Nek2 centrosomal kinase. Journal of Biological Chemistry 282: 6833-6842. PubMed

Wu, W., Baxter, J.E., Wattam, S.L., Hayward, D.G., Fardilha, M., Knebel, A., Ford, E.M., da Cruz e Silva, E.F. and Fry, A.M. (2007) Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase. Journal of Biological Chemistry 282: 26431-26440. PubMed.

Haque, F., Lloyd, D.J., Smallwood, D.T., Dent, C., Shanahan, C.M., Fry, A.M., Trembath, R.C. and Shackleton, S. (2006) SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton. Molecular and Cellular Biology 26: 3738-3751. PubMed.

Hayes, M.J., Kimata, Y., Wattam, S.L., Lindon, C., Mao, G., Yamano, H. and Fry, A.M. (2006) Early mitotic degradation of Nek2A depends on Cdc20-independent interaction with the APC/C. Nature Cell Biology 8: 607-614. PubMed.

Hames, R.S., Crookes, R.E., Hayes, M.J., Straatman, K.R., Merdes, A., Faragher, A.J. and Fry, A.M. (2005) Dynamic recruitment of Nek2 kinase to the centrosome involves microtubules, PCM-1 and localized proteasomal degradation. Molecular Biology of the Cell 16: 1711-1724. PubMed.

Mercer, K., Giblett, S., Oakden, A., Brown, J., Marais, R. and Pritchard, C. (2005)
A-Raf and Raf-1 work together to influence transient ERK phosphorylation and G1/S cell cycle progression. Oncogene 24:5207-5217. PubMed.

Mercer, K., Giblett, S., Green, S., Da Rocha Dias, S., Plumb, M., Marais, R. and Pritchard, C. (2005) Expression of endogenous oncogenic V600EB-raf induces proliferation and developmental defects in mice and transformation of primary fibroblasts. Cancer Research 65: 11493-11500. PubMed.

Shackleton, S., Smallwood, D.T., Clayton, P., Wilson, L.C., Agarwal, A.K., Garg, A. and Trembath R.C. (2005)
Compound heterozygous ZMPSTE24 mutations reduce prelamin A processing and result in a severe progeroid phenotype. Journal of Medical Genetics 42: 36-42. PubMed.

Shu, X., Fry, A.M., Tulloch, B., Manson, F.D.C., Crabb, J.W., Khanna, H., Faragher, A.J., Lennon, A., He, S., Trojan, P., Giessl, A., Wolfrum, U., Swaroop, A., Vervoot, R. and Wright, A.F. (2005) RPGR ORF15 isoform co-localises with RPGRIP1 at centrioles and basal bodies and interacts with nucleophosmin. Human Molecular Genetics 14: 1183-1197. PubMed.

Rapley, J., Baxter, J.E., Wattam, S.L., Casenghi, M., Meraldi, P., Nigg, E.A. and Fry, A.M. (2005) Coordinate regulation of the mother centriole component Nlp by Nek2 and Plk1 protein kinases. Molecular and Cellular Biology 25: 1309-1324. PubMed.

Wilson, G.M., Fielding, A.B., Simon, G.C., Yu, X., Andrews, P.D., Hames, R.S., Fry, A.M., Peden, A.A., Gould, G.W. and Prekeris, R. (2005) The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. Molecular Biology of the Cell 16: 849-860. PubMed.

 

Publications arising from the Wellcome Trust Imaging grant (060514) 2004-2000.

Hayward, D.G., Clarke, R.B., Faragher, A.J., Pillai, M.R., Hagan, I.M. and Fry, A.M. (2004) The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer. Cancer Res. 64:7370-7376. PubMed.

Mistry, P., Deacon, K., Mistry, S., Blank, J. & Patel, R. (2004) NF-kB promotes survival during mitotic cell cycle arrest. J. Biol. Chem. 279: 1482-1490. PubMed.

Pritchard, C.A., Hayes, L., Wojnowski, L., Zimmer, A., Marais, R. & Norman, J.C. (2004) B-Raf acts via the ROCK II/LIMK/cofilin pathway to maintain actin stress fibres in fibroblasts. Mol Cell Biol. 24: 5937-5952. PubMed.

Roberts, M.S., Woods, A.J., Dale, T.C., Van Der Sluijs, P. and Norman, J.C. (2004) Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins. Mol Cell Biol. 24: 1505-1515. PubMed.

Romio, L., Fry, A.M., Winyard, P., Malcolm, S. Woolf, A. and Feather, S. (2004) OFD1 encodes a centrosomal/basal body protein expressed during mesenchymal-epithelial transition in human organogenesis. J. Am. Soc. Nephrol. 15:2556-68. PubMed.

Twomey, C., Wattam, S.L., Pillai, M.R., Rapley, J., Baxter, J.E. and Fry, A.M. (2004) Nek2B stimulates zygotic centrosome assembly in Xenopus laevis in a kinase-independent manner. Dev. Biol. 265: 384-398. PubMed.

Warnke, S., Kemmler, S., Hames, R.S., Tsai, H-L., Hoffmann-Rohrer, U., Fry, A.M. and Hoffmann, I. (2004) Polo-like kinase-2 is required for centriole duplication in mammalian cells. Curr. Biol. 14: 1200-1207. PubMed.

Woods, A.J., White, D.M., Caswell, P.T., and Norman, J.C. (2004) PKD/PKCm promotes avb integrin recycling and delivery to nascent focal adhesions. EMBO J. 23: 2531–2543.PubMed.

Deacon, K., Mistry, P., Chernoff, J., Blank, J. and Patel, R. (2003)
. p38 MAP kinase mediates cell death and p21-activated kinase (PAK) mediates cell survival during chemotherapeutic drug-induced mitotic arrest. Mol. Biol. Cell. 14: 2071-2087. PubMed.

Faragher, A.J. and Fry, A.M. (2003) Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles. Mol. Biol. Cell 14: 2876-2889. PubMed.

Giannone, G., Jiang, G., Sutton, D.H., Critchley, D.R. and Sheetz, M.P. (2003)
Talin1 is critical for force-dependent reinforcement of initial integrin-cytoskeleton bonds but not tyrosine kinase activation. J. Cell Biol. 163: 409-419. PubMed.

Hagemann, C., Patel, R. and Blank, J. (2003).
MEKK3 interacts with the PA28 gamma regulatory subunit of the proteasome. Biochem. J. 73: 71-79. PubMed.

Roberts, M.S., Woods, A.J., Shaw, P.E. and Norman, J.C. (2003)
ERK1 associates with alpha(v)beta 3 integrin and regulates cell spreading on vitronectin. J Biol Chem, 278: 1975-1985. PubMed.

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