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In vivo confocal images of a two day old zebrafish labelled to identify presynaptic (green, upper image, SV2 antibodies) and postsynaptic (red, centre image, rhodamine-conjugated alpha-bungarotoxin) components of neuromuscular synapses. Putative synapses are revealed where the two stains colocalize (yellow, lower image).
Images taken by Sophie Bradley using the Olympus FV1000 confocal laser scanning microscope. Project leader:
Dr Jonathan McDearmid
, Department of Biology.
Kidney proximal tubular cells over-expressing CD36 were incubated with FITC-albumin (green) at 4°C, then stained for CD36 (red) and DNA (blue) . When the images were merged CD36 and FITC-albumin were seen to co-localise (yellow). Project of Professor Nigel Brunskill/Dr Richard Baines, stained by Dr Ravinder Chana. Department of Infection, Immunity and Inflammation. Imaged by Dr Kees Straatman (CBS) on the Leica SP5 confocal laser scanning microscope.
Interpahse CHO cell stained for gamma-tubulin (green), alpha-tubulin (red) and DNA (blue). The centrosome is labelled with both gamma-tubuline and alpha-tubulin and is therefore yellow in the image. Image taken on a Nikon TE300 inverted microscope by Dr. Suzy Prosser. Project group
Professor Andrew Fry, Department of Biochemistry..
Pecan antibody staining on 9½ day old mouse embryo Maximum intensity projection of the blood vessel network in a 9½ days old mouse embryo using Pecam1 antibody (CD31). Imaged on the Leica TCS SP5 using tiled scanning (composition of 4 images) and further enhanced using medium 3D filter from the LASAF software. Project of -00000000 Professor David Critchley/Dr. Sue Monkley, stained by Lorraine Spence. imaged by Dr. Kees Straatman. Department of Biochemistry.
Talin1 staining on MEF Maximum intensity projection of a MEF cell labeled with antibody for Talin1 (green), actin (red) and DNA (b lue). Imaged on the Leica TCS SP5. Project of Professor David Critchley/Dr.Kees Straatman. Department of Biochemistry.
Cilia Maximum intensity projection of human nasal epithelial cell at d19 of being at an air-liquid-interface (ALI culture). Transfer of cells to ALI stimulates them to differentiate and undergo cilioge nesis. Staining is red C-Nap1 (basal bodies/centrioles), green acetylated tubulin (cilia) and blue Hoechst (DNA). The samples were prepared by Dr Rob Hirst in collaboration with Professor Andrew Fry and Dr. Suzy Prosser. Imaged by Dr. Suzy Prosser on the Leica SP5 confocal laser scanning microscope. Department of Biochemistry
Cilia Same cells as above but  now a z-stack reconstruction and viewed in xz to measure cilia length. Imaged by Dr. Suzy Prosser on the Leica SP5 confocal laser scanning microscope. Department of Biochemistry.
cell cycle stages Different stages of the cell cycle. Maximum intensity projection of CHO cell labelled for gamma-tubulin (green) and alpha-tubulin (red). DNA is stained using Hoechst (blue). Dr. Suzy Prosser/Professor Andrew Fry. Imaged by Dr. Suzy Prosser on the Leica SP5 confocal laser scanning microscope. Department of Biochemistry.
clock neurons in Drosophila Maximum intensity projection of a Drosophila brain showing in green LacZ nuclear reporter expressed in clock neurons and glia and in red PDF, a neuropeptide, expressed in a subset of cl ock neurons, the small and large lateral neurons ventral. Imaged by Dr. Ezio Rosato on the Leica SP5 confocal laser scanning microscope. Department of Genetics.

photoreceptors in Drosophila brain

Maximum intensity projection of a Drosophila brain showing photoreceptors expressing Rhodopsin 6 in green and in red PDF, a neuropeptide, expressed in a subset of clock neurons, the small and large lateral neurons ventral. Imaged by
Dr. Ezio Rosato on the Leica SP5 confocal laser scanning microscope. Department of Genetics.

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