Fluorescent vectors available via PROTEX

Below is a list of vectors available via PROTEX to tag your protein of interest for use with fluorescence microscopy or bioluminescence imaging.

Vector NameARAffinity tagProtease cleavageHost/system
pLEICS-12
Amp/Neo
N-HIS10/3 x Flag
TEV Extra GA
Mammalian cells
pLEICS-13
Amp/Zeo
N-HIS10/3 x Flag
TEV Extra GA
Mammalian cells
pLEICS-21
Kan/Neo
N-EGFP
TEV Extra GA
Mammalian cells
pLEICS-22
Kan/neo
N-EYFP
TEV Extra GA
Mammalian cells
pLEICS-23
Kan/Neo
N-mCherry
TEV Extra GA
Mammalian cells
pLEICS-24
Kan/Neo
N-ECFP
TEV Extra GA
Mammalian cells
pLEICS-25
Kan/neo
N-mEGFP (A206K)
TEV Extra GA
Mammalian cells
pLEICS-26
Kan/Neo
N-mEYFP (A206K)
TEV Extra GA
Mammalian cells
pLEICS-27
Kan/Neo
N-mECFP (A206K)
TEV Extra GA
Mammalian cells

pLEICS-29

Kan/Neo

C-EGFP

TEV, Extra ENLYFQ

Mammalian cells

pLEICS-30

Kan/Neo
C-mCherry

TEV, Extra ENLYFQ

Mammalian cells

pLEICS-39

Amp/Neo

N-His103 x Flag, C-Halo

TEV, Extra GA at N-terminal Mammalian cells

pLEICS-40

Amp/Neo

N-His103 x Flag, C-hBirA

TEV, Extra GA at N-terminal Mammalian cells

pLEICS-45

Amp

N-HIS6/mEGFP

TEV Extra GA

E. coli DE3*

pLEICS-85

Amp

Luciferase

Mammalian cells

pLEICS-51

Kan

N-HIS6-N-FlAsH

TEV Extra SM

E. coli DE3*

pLEICS-52

Kan

N-HIS6-N-FlAsH/mEGFP128

TEV Extra SM

E. coli DE3*

pLEICS-53

Kan

N-HIS6-N-FlAsH/mEGFP129

TEV Extra SM

E. coli DE3*

pLEICS-54

Kan

N-HIS6/N-mEGFP128

TEV Extra SM

E. coli DE3*

pLEICS-55

Kan

N-HIS6/N-mEGFP129

TEV Extra SM

E. coli DE3*

pLEICS-56

Kan

Super-mEGFP1-215

TEV Extra SM

E. coli DE3*

pLEICS-57

Kan

N-HIS6/Super-mEGFP216-232

TEV Extra SM

E. coli DE3*

pLEICS-58

Amp

mEGFP128

TEV Extra SM

E. coli DE3*

pLEICS-59

Amp

mEGFP129

TEV Extra SM

E. coli DE3*

pLEICS-60

Kan

N-HIS6-FlAsH Super-mEGFP1-215

TEV Extra SM

E. coli DE3*

pLEICS-61

Amp

N-HIS6-FlAsH

TEV Extra SM

E. coli DE3*

pLEICS-63

Amp

C-Super folded mEGFP-HIS6

TEV Extra ENLYFQ

E. coli DE3*

pLEICS-64

Amp

C-mEGFP-HIS6

TEV Extra ENLYFQ

E. coli DE3*

pLEICS-65

Amp

C-Super folded mEGFP1-10/HIS6

TEV Extra ENLYFQ

E. coli DE3*

pLEICS-66

Amp

C-Super folded mEGFP11/HIS6

TEV Extra ENLYFQ

E. coli DE3*

pLEICS-67

Amp

N-HIS6/mCherry

TEV Extra GA

E. coli DE3*

pLEICS-68

Amp

N-HIS6/mEYFP

TEV Extra GA

E. coli DE3*

pLEICS-72

AMP EGFP Stem cell

pLEICS-73

AMP EGFP Stem cell

pLEICS-85

Amp

Luciferase

Mammalian cells

pLEICS-86

Amp

Luciferase/enhancer

Mammalian cells

pLEICS-87

Amp

Luciferase/Promoter

Mammalian cells

pLEICS-88

AMP mCherry, EGFP Stem cell

pLEICS-94

Amp C-EGFP Retroviruses

*E. coli DE3 hosts MUST express T7 RNA polymerase

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