Scanning large images using NIS-Elements

You can image areas larger than you field of view (FOV), the only caveat is that you can’t do this from the ND acquisition menu meaning it can’t be done in combination with a time-lapse experiment. If you want to include time-lapse it is possible to set the area using this menu and then transfer the information (e.g. if it recommends 2x4 FOV’s) to the ND acquisition menu.

There are two options

Go to the acquire menu and select 'scan large image', this will bring up a floating GUI as below:

Imaging large areas

1. In the box with the title Area you will see a drop down menu, this will either say 'Number of Fields in X and Y' or 'Left, Top, Right and Bottom Limits' ; select: 'Left, Top, Right and Bottom Limits'. Four boxes containing arrows will appear as indicated in the image above. Click on the green arrowhead live button in the bottom left hand corner to get a live image on the screen. You have to physically move the stage, looking at the live image on the screen (main live window, not the window panel in the menu), to the top of your sample and click the arrow facing up, move the stage to the far left of your sample and click the arrow facing left etc…  it will then update the information at the bottom of the area section to show how many fields you require and how big (in mm) your large image will be.

Take care that you select the correct objective you want to use for the scan in the 'Capture' area above the arrows. The menus on the right allow you to include Z-series and multi-channel imaging and will allow you to set up autofocus if required. Menus at the bottom allow you to save the results directly to file.

2. A second option is if you want to scan a large image at high magnification and your sample will fit into a full FOV at a lower power objective you can use the capture macro image function – for example if you want to scan at 100x and your sample will fit into a single FOV at 10x you can use the Capture button (see circle in image below) in the “Stage Overview with Macro Image” section of this menu and draw around the area you want to image – just ensure that the boxes in the top left corner (red box) are set correctly – in this example Macro Image would have the objective set to 10x and Scanning set to 100x.

NIS2

The menus on the right allow you to include Z-series and multi-channel imaging and will allow you to set up autofocus if required. Menus at the bottom allow you to save the results directly to file.

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