Olympus Objectives

In the Adrian Building three Olympus microscopes were installed in 2007, funded by the Wolfson Foundation.

Below is a list of objectives available for the different Olympus microscopes. Please contact me (krs5) in advance if you need an objective that is not installed on the microscope system you want to use.

systemMagOilNADescriptionGradeWD(mm)Coverglass
Correction
Cytological 10x - 0.3 UPlanFLN C 10 -
Cytological 20x - 0.5 UPlanFLN C 2.1 0.17
Cytological 40x + 1.3 UPlanFLN C 0.2 0.17
Cytological 60x + 1.35
UPlanSAPO A 0.15 0.17
Cytological 100x + 1.4 UPlanSAPO A 0.13 0.17
CellR/SCanR 4x - 0.13 UPlanFLN 17 0.17
CellR/SCanR 10x - 0.4 UPlanSAPO A 3.1 0.17
CellR/SCanR 20x - 0.45 LUCPlanFLN B 6.6-7.8 0-2
CellR/SCanR 40x - 0.6 LUCPlanFLN B 2.7-4.0
0-2
CellR/SCanR 40x + 0.65-1.35 UAPO F 0.1 0.17
CellR/SCanR 60x - 0.7 LUCPlanFLN B 1.5-2.2 0.1-1.3
CellR/SCanR 60x + 1.42 PlanAPON A 0.15 0.17
CellR/SCanR 100x + 1.4 UPlanSAPO A 0.13 0.17
confocal FV1000 10x - 0.4 UPlanSAPO A 3.1 0.17
confocal FV1000 20x - 0.75 UPlanSAPO A 0.6 0.17
confocal FV1000 40x + 1.3 UPlanFLN C 0.2 0.17
confocal FV1000 60x + 1.35 UPlanSAPO A 0.15 0.17

* The 60x long working distance objective for the Cell^R/Scan^R system is extra and NOT installed on the system.

Grade Description
A High-performance universal plan wide-range apochromatic-corrected objectives, corrected for four colors chromatically and spherically, with unsurpassed numerical aperture values deliver the best resolution, contrast and field flatness for any microscope technique. In combination with the lowest autofluorescence of all objective series, they are the best choice for advanced fluorescence applications.
B Semi-apochromatic long working distance objectives, designed for tissue culture observations in flasks and dishes. Excellent contrast and resolution in brightfield, phase contrast, DIC and fluorescence observations. Corrected for spherical and chromatic aberration at two wavelengths (green and red)
C semi-apochromatic universal objectives that deliver superb resolution, contrast and image flatness with any microscopic technique. Corrected for spherical and chromatic aberration at two wavelengths (green and red)
F High transmission in the near-UV wavelength (340 nm) makes these objectives perfect for applications such as ratio imaging or measurement of intracellular pH with UV-excitable fluorochromes.

More information about the objectives can be found the Olympus website

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