Zeiss MP7 multiphoton microscope


Location: Maurice Shock Building, room 383.
Contact:
Mr Matthew Barker (mcb8/3085)
Dr Kees Straatman (krs5/7085/2263)

Multiphoton systems are used to image deeper into the sample. Using a normal confocal laser scanning microscope images can be taken up to 50 µm into the sample. This system should be able to image to a depth of around 1 mm.

 

This microscope is only for multiphoton imaging and is equipped with a Mai Tai eHP DS Ti:Sapphire pulsed laser (< 70 fs pulsewidth) with DeepSee dispersion. The laser is tunable between 690-1040nm.
The system is build around an upright Axio Examiner.Z1 microscope and has two non-descanned detectors (NDD) and a transmitted light detector.

 

One W-Plan-Apochromat 20x/NA=1 DIC(UV) VIS-IR water dipping objective is available. It is apochromatically corrected from VIS to IR and designed for use without cover slip.

 

Improvement by use of DeepSee compensation (images Newport Spectra-Physics Ltd):

Deepsee1Deepsee2

Image left is without compensation, image right is with Dispersion Compensation
Both images at 800 nm, same power on the sample (~2 mW) and same PMT gain settings.

 

Filters available for the two NDDs:
mirror + SP485 nm
mirror + BP 455-500 nm
mirror + BP 500-550 nm
LP490 nm + BP 500-550 nm
LP510 nm + BP520-560 nm
LP555 nm + BP 565-610 nm

 

Literature

 

Two-photon absorption properties of fluorescent proteins (2011) M. Drobizhev, N.S. Makarov1, S.E. Tillo, T.E. Hughes and A. Rebane1. Nat. Meth. 8: 393-399.
A new approach to dual-color two-photon microscopy with fluorescent proteins (2010) S.E. Tillo, T.E. Hughes, N.S. Makarov, A. Rebane, M. Drobizhev. BMC Biotechnol. 10: 6-12.

 

 

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