Zeiss LSM980 Airyscan 2

Location: HWB room 3/51
Contact: Dr Kees Straatman (krs5/7085, 2263)


Basic user guide
Multiplex Mode of ZEISS LSM 980 Airyscan 2 (YouTube)

Early 2020 we have introduced the Zeiss LSM 980 Airyscan 2 system in the facility. This microscope was funded by the BBSRC ALERT18 equipment round.

 

When publishing results obtained using the Zeiss LSM980 Airyscan 2 confocal laser scanning microscope you are required to acknowledge the BBSRC grant nr: BB/S019510/1


UKRI-BBSRC-transp

Laser lines available:

405nm
445nm
488nm
514nm
561nm
639nm

 

LSM980Airyscan SRMultiplex SR-4YMultiplex SR-8YMultiplex CO-8Y

Parallelization

1 4 8 8
Resolution (nm) 120/120 140/140 120/160 confocal or better
Max fps at max Field of view 0.2 (zoom 1,7) 1 (zoom 1) 2 (zoom 1) 9.6 (zoom 1)
Max fps 512x512 4.7 25 47.5 34.4
ab fine structure +++++ ++++ +++ ++
ab tiling ++ ++++ ++++ ++
Live cell imaging ++ +++ ++++ +++++

Further improvement of resolution up to around 40 nm can be obtained using FEAST imaging (fluctuation-enhanced Airyscan technology). This uses SRRF on airyscan images. see Wang et al. (2020) Multicomposite super-resolution microscopy: Enhanced Airyscan resolution with radial fluctuation and sample expansions. e2419. doi: 10.1002/jbio.201960211

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