5 Ways Opal™ Powers Your Spatial Biology Studies

1. It's accessible
Similar to standard immunohistochemistry, Opal™ Multiplex IHC kits make multiplex results accessible to anyone who works with standard IHC — enabling practical and reliable multiplexed staining and analysis of up to eight markers plus DAPI counterstain.

Video | Opal™ Multiplex IHC Assay Development and Optimization Tutorial

2. Make autofluorescence a thing of the past
Opal reagents utilize tyramide signal amplification (TSA) to increase antigen marker intensity by 10-100 fold compared to conventional indirect immunofluorescence methods, reducing autofluorescence when used in conjunction with multispectral imaging

Webinar | Autofluorescence: What Lies Beneath

3. Use the best primary antibodies — with no cross-reactivity
Opal fluorophores are compatible with any species, so you can select antibodies based on performance.

Technical Brief | Primary Antibodies and Opal Pairings for Multiplex IHC

4. Focus on data, not development
Akoya provides the only end-to-end solution for whole slide tissue multiplexing with validated parameters for staining, imaging, and analysis. Try their MOTiF PD-1/PD-L1 panel kits for detection and quantification of up to six validated antibodies in a single tissue section.

Technical Note | MOTiF™ Panel Kits: Turnkey Validated Opal® Staining and inForm® Analysis Using Vectra Polaris®

Webinar | Just Add Tissue: The Ready-to–Use Phenoptics Workflow

5. Support every step of the way
Akoya provides comprehensive training and support, to help you to stain, image, and analyze with confidence.

© 2019 Akoya Biosciences, Inc. All rights reserved. Akoya Biosciences, CODEX, inForm and Vectra are registered trademarks and  Mantra, Opal, Phenoptics, Polaris and The Spatial Biology Company are trademarks of Akoya Biosciences, Inc. A Delaware corporation.

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