Advanced Imaging Facility (AIF)

Advance Imaging Facility

Check additional COVID-19 rules for use of AIF

Watch the AIF COVID-19 video

The Advanced Imaging Facility (AIF) is the open access light microscopy facility of the College of Life Sciences and offers a wide range of confocal and conventional fluorescence microscopes and image analysis resources. The AIF is part of the Centre for Core Biotechnology Services (CBS) and it forms together with the Electron Microscopy Facility, Pre-Clinical Imaging Facility and Histology Facility the core of the imaging facilities in the college. Dr Kees Straatman is manager of the AIF.

The AIF has annually over 100 microscope users and is open to all users of the University of Leicester, but also members of other M5 Universities (Birmingham, Aston, Warwick, Nottingham and Loughborough) and other academic institutions are welcome.

Focus on:

• Fluorescence, brightfield, DIC and bioluminescence imaging
• 2D/3D/4D imaging
• Live cell imaging
• Multi-colour fluorescence imaging
• Multi-position imaging
• Spectral imaging
• High throughput slide scanning/digital pathology
• F-techniques (FRAP, FLIP, FRET and FCS)
• High content screening
• Metaphase finding
• Correlative Light and Electron Microscopy (CLEM), in collaboration with the EM Facility
• Immunohistochemistry colour imaging
• Quantitative imaging
• Image analysis and processing (ImageJ/Fiji macros, Imaris, Volocity, Huygens deconvolution)
• Data storage
• Training and teaching
• Advice

Within the AIF we try to provide users with easy access to advanced imaging technologies but microscopy is continuously and rapidly evolving and several novel imaging techniques or improvements have recently become commercially available. Besides this, several research groups within the College build or built their own microscope systems. How can we improve microscopy in the AIF and in the College in general? What additional system would you like to see included in the AIF? Please visit the future.

If you publish results obtained using the AIF please mention the AIF in your acknowledgements and send a reference to us. This will make securing of future funding for the AIF easier. View our list of publications.


Next workshops:
'Introduction to ImageJ' -  Monday 8th of June - Cancelled
'Writing macros in ImageJ' -  Tuesday 9th of June - Cancelled

The AIF would like to thank the following funding bodies for their generous funding:

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