Universal species identification in the field by rapid and affordable nanopore DNA sequencing

Determining species of origin from biological traces is often achieved by reading “barcode” genes in mitochondrial DNA which differ in sequence between species. Currently DNA sequencing must be performed in a laboratory due to the size and complexity of the equipment and the manual stages required to prepare the DNA for analysis. Third-generation nanopore sequencing will allow this to be done at a suspected crime scene yielding a species identification in an hour rather than days, with minimal training. This would allow rapid detection and arrest which would have both a deterrent effect and raise public awareness.

This project, supported by a $10K USAID Wildlife Crime Tech Challenge prize to Jon Wetton, and in partnership with Oxford Nanopore Technologies, aims to develop a single hand-portable device to test stains in rhino poaching cases, identify bushmeat and detect substitution of protected species in the caviar trade among many other possible applications.

 

See our video here 

Wildlife Conservation – University of Leicester

https://www.youtube.com/watch?v=m5dCA54pUQE

Please forward any enquiries regarding the Wildlife Crime Tech Challenge to Jon Wetton (jw418@le.ac.uk) or Celia May (cam5@le.ac.uk).

Share this page:

Contact us

Contact us at forensicscience@le.ac.uk

Latest News

Follow INTREPID Forensics PhD student research blogs here.

Blazing Car Murder victim still remains a mystery.  Click here for the press release.  View the film here.