Dr Martha Clokie

Reader 

Contact details

Lab: 105, MSB / Office: 106, MSB

Telephone: (+44) (0)116 252 2959

E-mail: mrjc1@le.ac.uk 

Research Interests

  • Ecology and molecular biology of bacteriophages and their relationships with bacterial hosts; ranging from bacterial pathogens to environmentally important cyanobacteria.
  • Exploitation of bacteriophages and phage-derived products as an alternative to treating antibiotic resistant bacterial infections e.g Clostridum difficle infections.
  • Sequencing and annotation of newly isolated cyanophages. Establishing the function of key cyanophage genes to link gene function to ecology of the organisms and investigation the potential to exploit unique enzymes found in cynophage genomes. 
  • Further Research pages
  • Publications

Selected Recent Publications

Hargreaves KR, Kropinski A, Clokie, MRJ. What does the talking? (2014): Quorum sensing signalling genes discovered in a bacteriophage genome. PLoS ONE; 7:   DOI: 10.1371/journal.pone.0085131

Traving, S., Clokie, MRJ,*  Middelboe, M* (joint last authors) (2014). Increased acidification has a profound effect on the interactions between the cyanobacterium Synechococcus sp. WH7803 and its viruses. FEMS Microbiology Ecology. Increased acidification has a profound effect on the interactions between the cyanobacterium Synechococcus 87:133-141.

 Hargreaves KR, Colivn HV, Patel KV, Clokie JJ, Clokie MRJ (2013). The estuarine environment has genetically diverse Clostridium difficile which harbour abundant prophages, Appl. Environ. Microbiol. 79: 6236-6243.

 Malik, DJ, Patel KV, Clokie MRJ and Shama G (2013).  On the difficulties of isolating Clostridium difficile from hospital environments.  Journal of Hospital infection 84:181-183.

 Adriaenssens E.M., Lehman SM, Vandersteegen K, Vandenheuvel D, Philippe DL, Cornelissen A, Clokie, MRJ, Garcı´AJ, De Proft M, Maes, M, Lavigne R (2012).  CIMs monolithic anion-exchangechromatography as a useful alternative to CsCl gradientpurification of bacteriophage particles. Virology.  DOI:10.1016/j.virol.2012.09.018.

Hargreaves KR, Anderson NJ, Clokie MRJ. Recovery of viable cyanophages from the sediments of a eutrophic lake at decadal timescales. FEMS Microbiology Ecology 2012:DOI:10.1111/1574-6941.1200

Shan J, Patel KV, Hickenbotham PT, Nale JY, Hargreaves KR, Clokie MRJ. Prophage carriage and diversity within clinically relevant strains of Clostridium difficile. Applied and Environmental Microbiology 2012;78:6027-34

Nale JY, Shan J, Hickenbotham PT, Fawley WN, Wilcox MH, Clokie MRJ. Diverse temperate bacteriophage carriage in Clostridium difficile 027 strains. PLoS ONE 2012;7:DOI.10.1371/journal.pone.0037263

Ray JL, Dondrup M, Mohda S, Steen IH, Saanda RA, Clokie MRJ. Finding the needle in the metagenomic viral haystack - micro-metagenome analysis captures a snapshot of the evolution of a bacteriophage armoire. PLoS ONE 2012;7:DOI.10.1371/journal.pone.0034238

Gatedee J, Kritsiriwuthinan K, Galyov E,  Shan J, Dubinina E, Intarak N, Clokie MRJ, Korbsrisate S. Isolation and characterization of a novel podovirus which infects Burkholderia pseudomallei. Virology Journal 2011;8:DOI: 10.1186/1743-422X-8-366

Chan Y-W, Mohr R, Millard AD, Holmes AB, Larkum AW, Whitworth AL, Mann NH, Scanlan DJ, Hess WR, Clokie MRJ. Discovery of cyanophage genomes which contain mitochondrial DNA polymerase.  Molecular Biology and Evolution 2011;8:2269-74

Clokie MRJ, Millard AD, Mann NH. T4 genes in the marine ecosystem: Studies of theT4 like cyanophages and their role in marine ecology. Virology Journal 2010;7:291

Jia Y, Shan J, Millard AD, Clokie MRJ, Mann NH. Light-dependent adsorption of photosynthetic cyanophages to Synechococcus sp. WH7803.  FEMS Microbiology Letters 2010;310:120-6

Millard AD, Gierga G, Clokie MRJ, Evans DJ, Hess WR, Scanlan DJ.  An antisense RNA in a lytic cyanophage links psbA to a gene encoding a homing endonuclease. The ISME Journal 2010;1–15

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Contact Details

Department of Infection, Immunity and Inflammation,
Maurice Shock Medical Sciences Building,
University Road,
Leicester, LE1 9HN, UK 

General enquiries to:
Alison Brown

T:+44 (0)116 252 2951
F:+44 (0)116 252 5030

Postgraduate enquiries to: 
Susan Kapadia (PhD)

Frances Tarte (MSc)

Erasmus and Study Abroad Co-ordinator:

Professor Peter Andrew

T: +44 (0)116 252 2951

Other contact details can be found in People

Head of Department: Professor Peter Andrew