Reagent Production Methods
The recipes below are in use on laboratories on level 3 of the RKCSB. Queries about their use should be directed to Angie Gillies (akg8@leicester.ac.uk) in the first instance.
- 100bp ladder
- A salts 5x
- AJ 10x
- AJ 10x for Ass. PCR
- Amersham wash buffer
- Ammonium acetate 3M
- 10% Ammonium Persulphate
- Ammonium sulphate 1M
- Ampicillin 100mg/ml
- Annexin V buffer
- Avidin (ELISA)
- bind and wash buffer 2x
- Blocking Buffer for Survivin
- Blocking solution
- 5-Bromo-2’Deoxyuridine (BrDU)
- Bromophenol blue 1%
- CaCl2 1M
- Calcium chloride 1M
- Calibrating pH meter
- Chloroform/IAA
- Citrate buffer 10mM
- Citrate buffer 20x
- Cova buffer
- DAB
- DEA 1M
- Denaturing solution
- Denhardts
- DEPC water
- Depurinating solution
- Destaining solution
- Development buffer
- Dextran sulphate 50%
- Dialysis tubing
- Dithiotheitol (DTT) 1M
- DNA Extraction Buffer
- dNTP stock 10mM
- DYNABEADS solutions
- EDTA 0.5M
- Eosin
- Ethanol/DEPC 70%
- Ethidium bromide
- Formal saline 10%
- paraFormaldehyde
- Gel loading buffer
- Glucose 20%
- Gold lysis buffer
- Hanahan 1 litre
- H broth agar
- H soft top agar
- L Broth / Agar / Top agar
- Levamisole 1M
- poly-D-Lysine stock solution
- HCl dilutions
- M9 salts
- MgCl2 1M
- Maleic acid buffer
- Mayer’s Haematoxylin
- Methacarne Fixative
- Michele’s neutralising solution
- Minimal media
- MOPS 10x
- MnCl2 1M
- NaCl 1M / 5M
- NaOH 5M
- NRS
- Neutralising solution
- NHS-Biotin (ELISA)
- Nuclear fast red
- OLB (In-Situ)
- OLB (Southern blotting)
- PBS 10x / PBS concentrate
- PE 5x
- PE Mod. 10x (In-Situ)
- PEG 36%
- pH buffers
- PhiX 174 / HaeIII
- Protease
- Proteinase K
- Rapid DNA extraction buffer
- REACT 1
- REACT 2
- REACT 3
- REACT 4
- REACT 10
- Renaturation buffer
- Resolving Buffer for polyacrylamide gels (1.5M Tris-HCl pH 8.8)
- RNASE A
- Running buffer 10x
- S.M.
- Sample buffer
- SDS 10%
- SDS-Page electrode buffer
- SDS-Page gel buffers
- Semi-dry transfer buffer
- Sephadex G50
- Silane slides
- Silane slides (no acetone)
- Siliconised coverslips
- Silver stain fixer
- Silver nitrate stock and working solutions
- Silver stain developer
- Silver stain neutralizer stock and working solutions
- Spermidine
- SOB medium
- Sodium acetate 2M
- Sodium acetate 3M
- Sodium azide 15mM (ELISA)
- Sodium borate 1M
- Sodium cacodylate 1.2M
- Sodium orthovanadate
- Solution 1 (plasmid prep.)
- Solution D / 1M Sodium citrate pH7 DEPC
- SSC 20x
- SSCP loading buffer
- ssDNA
- SSPE 20x
- Staining solution
- Sterile ultra-pure water
- Stop solution
- Subbed slides
- TAE
- TBS concentrate
- Tdt buffer
- TE
- Thiamine
- Tris/DEPC
- Tris/SDS Buffer for rapid extraction of DNA
- TBE 10x
- Tris 50mM / MgCl2 10mM
- Tris-Glycine (SDS-PAGE)
- Tris/HCl 1M
- Tris HCl pH6.8 0.5M
- UMFIX fixative for fresh tissue
- VAB
- Washing-up
- Wash solution for Tri-Reagent
- Western gel loading buffer 4x
- YT medium
