Dr Nina Storey
|Tel: 0116 229 7145 Email: email@example.com|
|1994||Biochemistry (Hons), University of Edinburgh|
|1999||PhD Molecular Pathology, University College London|
|1999-2004||Visiting Fellow, membrane signalling group, National Institute of Environmental Health Sciences, National Institute of Health, USA|
|2005-2007||Research Associate, Department of Cell Physiology and Pharmacology, University of Leicester|
|2007 - present||Lecturer, Department of Cell Physiology and Pharmacology, University of Leicester|
Research Interests and Techniques
Brief summary of current Research Interests
Ion channel modulation underlies many physiological processes. We study the fundamental properties of heart cells and ion channel modulation by intracellular signaling pathways that regulate cardiac myocyte function.
Fig1. Single cardiac myocyte
Major Areas of Research
- To investigate cardiac voltage-gated ion channel regulation by hormone signalling, including G protein signaling, rapid effects of steroid hormones, calcium and phosphorylation and heme.
- To understand the molecular mechanisms of cardiac protection focusing on the role and regulation of KATP ion channels in cardiac muscle.
- To understand the molecular processes of reperfusion injury, focusing on the central role of the mitochondria for reactive oxygen species production and mitochondrial permeability transition pore opening.
We take an integrated approach and the methods that we currently use include the following:
- Electrophysiology to record ion channel activity by recording action potentials or whole-cell currents and single channel currents
Fig2. Single KATP channel currents
- Single cell imaging to detect fluorescent indicators of intracellular calcium, reactive oxygen species, mitochondrial membrane potential, and other factors or GFP tagged recombinant proteins
- Molecular biology approaches to investigate the role and regulation of ion channels taking advantage of many types of constructs for up/down regulation of ion channel subunits and for structure function analysis
- Measurement of single cardiac myocyte contraction
Current Group Members
Dr Mark Burton
British Heart Foundation
SDF-1α and LPA modulate microglia potassium channels through rho gtpases to regulate cell morphology. Muessel MJ, Harry GJ, Armstrong DL, Storey NM. Glia. 2013
Mitochondrial ROS production and subsequent ERK phosphorylation are necessary for temperature preconditioning of isolated ventricular myocytes. Bhagatte Y, Lodwick D, Storey N. Cell Death Dis. 2012
Evidence that Ca2+ within the microdomain of the L-type voltage gated Ca2+ channel activates ERK in MIN6 cells in response to glucagon-like peptide-1. Selway J, Rigatti R, Storey N, Lu J, Willars GB, Herbert TP. PLoS One. 2012
Kir6.2 limits calcium overload and mitochondrial oscillations of ventricular myocytes in response to metabolic stress. Storey NM, Stratton RC, Rainbow RD, Standen NB, Lodwick D. Am J Physiol Heart Circ Physiol. 2013 Sep 6
Storey NM, Gentile G, Ullah H, Gentile S, Russo A, Muessel MJ, Erxleben C and Armstrong DL. (2006). Rapid signaling at the plasma membrane by a nuclear receptor for thyroid hormone. Proceedings of the National Academy of Sciences of the United States of America. 103: 5197–5201.
Storey NM, Gomez-Angelats M, Armstrong DL and Cidlowski J. (2003). Stimulation of Kv1.3 potassium channels by death receptors during Apotosis in Jurkat T lymphocytes. Journal of Biological Chemistry 278: 33319-33326.
Storey NM, O’Bryan JP and Armstrong DL. (2002). Rac and Rho mediate opposing hormonal regulation of ether-a-go-go-related potassium channel. Current Biology 12 :27-33.
Storey NM, Latchman DS and Bevan S. (2002) Selective internalization of sodium channels in rat dorsal root ganglion neurons infected with Herpes simplex virus-1. Journal of Cell Biology 158: 1251-1262.