Service and Ordering

Cloning Service

We provide four kinds of service.

1. Clone into pLEICS vectors(s) - choice of 75 vectors.

We will prepare PCR products from supplied templates and primers (if PCR fails we will perform one round of troubleshooting), and clone into pLEICS vector(s) of choice.

Normally, orders received by Friday 4:00pm should be ready to return before 5:00pm next Friday.

2. Clone into customer's vector(s) adapted for LIC method.

We will adapt the customer's vector(s) to LIC vector(s) with fast positive colony screen which will cost £168.00 per vector and will take 6-8 weeks. We will then prepare PCR products from supplied templates and primers (including vector based colony PCR primers).

Cost for subsequent cloning is the same as for option 1.

Normally, orders received by Friday 4:00pm should be ready to return before 5:00pm next Friday.

3. Clone into customer's vector(s).

You supply the vector(s) with a sequence and map, PCR templates, vector based PCR primers (for ligase free cloning method) and vector based colony PCR primers. We then prepare vectors and PCR products from supplied templates and primers.

(For >10 clones, it is cheaper to adapt your vector(s) to LIC vector(s))

Normally, orders received by Friday 4:00pm should be ready to return before 5:00pm on the third Friday.

To order please:
1) For cloning DOWNLOAD order form and email to xy13@le.ac.uk and/or ap22@le.ac.uk

2) For mutagenesis DOWNLOAD order form and email to xy13@le.ac.uk and/or ap22@le.ac.uk
3) Please bring primers, templates and vectors (pLEICS vectors are supplied by us) to HWB Rm. 1/45

4. Protein Expression Test Service

We now offer 1ml His-tagged protein expression trials in E. coli. You supply the expression constructs and we will transform them into BL21 (DE3) or BL21 (DE3)-Rosetta, or you supply the E. coli expression strains containing your constructs (must be IPTG inducible). The protein expression test will be carried out in a 1ml culture in 2ml 96-well deep block, and the results will be presented as an SDS-Gel picture of fractions of total protein, soluble protein, Ni column flow-through, wash and elution. Currently, we can do expression trials at either 37° or 20° C. We can provide commonly used media - LB, TB and SOB but if necessary you can supply your specific media.

Current cost:

£8.30 per test

This service will run once every 3 weeks. Normally, for orders received by Friday 4:00pm the results should be ready before 5:00pm  next Friday.

To order please:

1) DOWNLOAD the order form and email to xy13@le.ac.uk and/or ap22@le.ac.uk
2) Please bring samples and materials to HWB Rm. 1/45

Any particular requirements please see Dr. Xiaowen Yang x7202/7092/HWB Rm. 1/61 or Mr. Andrew Prescott.