Primer Design Guide
Primer design for pLEICS vectors is very simple:
1) Decide which vector family you want to use (A to K below)
2) Copy the appropriate 5' vector homology region for forward and reverse primers
3) Design the insert homology region according to your desired insert 15-25 bp (longer if AT rich, shorter if GC rich) ensure 8bp at 3' end are unique, and end (3') in a A or T.
We will provide advice for customers to design vector based primers for the cloning using their vectors
Family A vectors homology region:
N-ter: GTATTTTCAGGGCGCC...
C-ter: GACGGAGCTCGAATTTCA...
Family B vectors homology region:
N-ter: AGGAGATATACATATG...
C-ter-His or GST tag: GAAGTACAGGTTCTC...
C-ter-no tag: GAAGTACAGGTTCTCTCA...
Family C vectors homology region:
N-ter: TACTTCCAATCCATG...
C-ter: TATCCACCTTTACTGTCA...
Family D vectors homology region (with TEV site):
N-ter: GTATTTTCAGGGCGCC ...
C-ter: GTCGACTGCAGAATTtca ...
Family E vectors homology region (without TEV eite):
N-ter: TCCGGACTCAGATCT ...
C-ter: GTCGACTGCAGAATTtca ...
Family F vectors homology region:
N-ter: CGAAACGAGGAATTCTCGATG ...
C-ter: CAGGTTCTCACTAGT ...
Family G vectors homology region:
N-ter: GCTCGAGAATTCTTCG ...
C-ter: CAGGTTCTCACTAGT ...
Family H vectors homology region:
N-ter: TACGTAGAATTCTCGATG ...
C-ter: CAGGTTCTCACTAGT ...
Family I vectors homology region:
N-ter: TACGTAGAATTCTCG ...
C-ter: CAGGTTCTCACTAGT ...
Family J vectors homology region (with TEV site):
N-ter: GTATTTTCAGGGCGCC ...
C-ter: CAATGCCAATAGGATATCtca ...
Family K vectors homology region (without TEV site):
N-ter: TCCGGACTCAGATCT ...
C-ter: GTGGTGGTGGTGCTCGAGtca ...
Family 29T vector homology region (with TEV site):
N-ter: CTACCGGACTCAGATCTCGAGATG...
C-ter: CTGAAAATACAGGTTCTCGAG...
Family 29 vector homology region (without TEV site):
N-ter: CTACCGGACTCAGATCTCGAGATG...
C-ter: TACCGTCGACTGCATGAATTC...
Family 35 vector homology region:
N-ter: ATGGCCATGGAGGCCGAATTC...
C-ter: TCGACGGATCCCCGGGAATTC...
Family 36 vector homology region:
N-ter: GCCATGGAGGCCAGTGAATTC...
C-ter: ATGCCCACCCGGGTGGAATTC...
Family 49 vector homology region:
N-ter: ACCCAAGCTTGGTACCATG...
C-ter: AAAATACAGGTTCTCGAG...
Family 72 vector homology region:
N-ter: CATCATTTTGGCAAAGAATTCATG ...
C-ter: GGAGGGAGAGGGGCGGAATTCtca ...
Family 73 vector homology region:
N-ter: ACCTGTGGGTACCCGCTCGAGATG ...
C-ter: TATCGATACCGTCGACCTCGAGtca ...
Family 74 vectors homology region:
N-ter: TCTGCTACCACAGCCGCGGATCC ...
C-ter: TCCTTCACAAAGATCCTCTAGAtca...
Family 76 vector homology region:
N-ter: CATCATCAGACCACGCGGATTC ...
C-ter: TCCTTCACAAAGATCCTCTAGAtca ...
Family 77 vector homology region:
N-ter: CATCATTTTGGCAAAACC ...
C-ter: GACGGAGCTCGAATTtca ...
Family 79 vector homology region:
N-ter: TTCAGGCGGCCGCGGCTCGAGC ...
C-ter: TTCTCCTTTACTCATGGATCCtca ...
Family 81 vector homology region:
N-ter: CTGGAATTCCCGGGGATC ...
C-ter: CTGCAGGTCGACGGATCC ...
Family 82 vector homology region:
N-ter: GGTGGGATCGATTGGATC ...
C-ter: CTCGGTACCCGGGGATCC ...
Family 85 vector homology region:
N-ter: TATCGATAGGTACCGAGCTC ...
C-ter: TATCGCAGATCTCGAGCCCGGG ...
