Advanced Imaging Facility (AIF)

Advance Imaging Facility

The Advanced Imaging Facility (AIF) is the open access light microscopy facility of the College of Medicine, Biological Sciences and Psychology and offers a wide range of confocal and conventional fluorescence microscopes and image analysis resources. It forms together with Electron Microscopy Facility, Pre-Clinical Imaging Facility, Histology Facility and Flow Cytometry the Leicester Imaging Technologies (LITE) and is part of the Centre for Core Biotechnology Services (CBS). Dr Kees Straatman is manager of the AIF.

The AIF has annually over 100 microscope users and is open to all users of the University of Leicester, but also members of other M5 Universities (Birmingham, Aston, Warwick, Nottingham and Loughborough) and other academic institutions are welcome.

Focus on:

• 2D/3D/4D imaging
• Fluorescence, brightfield, DIC and bioluminescence imaging
• Live cell imaging
• Multi-colour fluorescence imaging
• Multi position imaging
• Spectral imaging
• Imaging of large tissue areas
• F-techniques (FRAP, FLIP, FRET and FCS)
• High content screening
• Slide scanning
• Correlative Light and Electron Microscopy (CLEM), in collaboration with the EM Facility
• Immunohistochemistry colour imaging
• Quantitative imaging
• Image analysis and processing
• Data storage
• Training and teaching
• Advice

Within the AIF we try to provide users with easy access to advanced imaging technologies but microscopy is continuously and rapidly evolving and several novel imaging techniques or improvements have recently become commercially available. Besides this, several research groups within the College build or built their own microscope systems. How can we improve microscopy in the AIF and in the College in general? What additional system would you like to see included in the AIF? Please visit the future.

If you publish results obtained using the AIF please mention the AIF in your acknowledgements and send a reference to us. This will make securing of future funding for the AIF easier. View our list of publications.


Next workshops:
'Introduction to ImageJ' -  Thursday 1 June.
'Writing macros in ImageJ' -  Friday 2 June.

The AIF would like to thank the following funding bodies for their generous funding:

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